ArticlePlant cell reports2003
Quantitative GFP fluorescence as an indicator of recombinant protein synthesis in transgenic plants.
Article in Plant cell reports, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 74 citations in OpenAlex.
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- Rapid fluorescent reporter quantification by leaf disc analysis and its application in plant-virus studies.Plant methods · 2014Article
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- An epifluorescent attachment improves whole-plant digital photography of Arabidopsis thaliana expressing red-shifted green fluorescent protein.AoB PLANTS · 2012Article
- Visualization of plant viral suppressor silencing activity in intact leaf lamina by quantitative fluorescent imaging.Plant methods · 2011Article
- Quantification of the brassinosteroid insensitive1 receptor in planta.Plant physiology · 2011Article
- An efficient Foxtail mosaic virus vector system with reduced environmental risk.BMC biotechnology · 2010Article
- Corpus callosum: a favorable target for rSFV-mediated gene transfer to rat brain with broad and efficient expression.Journal of molecular neuroscience : MN · 2010Article
- Visual selection allows immediate identification of transgenic rice calli efficiently accumulating transgene products.Plant cell reports · 2009Article
- Quantitative evaluation of six different viral suppressors of silencing using image analysis of transient GFP expression.Plant cell reports · 2009Article
- Extremely high-level and rapid transient protein production in plants without the use of viral replication.Plant physiology · 2008Article
- Pollen-mediated intraspecific gene flow from herbicide resistant oilseed rape (Brassica napus L.).Transgenic research · 2007Review
- Isolation of two highly active soybean (Glycine max (L.) Merr.) promoters and their characterization using a new automated image collection and analysis system.Plant cell reports · 2007Article
- Transformation and segregation of GFP fluorescence and glyphosate resistance in horseweed (Conyza canadensis) hybrids.Plant cell reports · 2007Article
- An efficient fungal RNA-silencing system using the DsRed reporter gene.Applied and environmental microbiology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The utility of green fluorescent protein (GFP) for biological research is evident. A fluorescence-based method was developed to quantify GFP levels in transgenic plants and protein extracts. Fluorescence intensity was linear with increasing levels of GFP over a range that encompasses transgene expression in plants by the cauliflower mosaic virus 35S promoter. Standard curves were used to estimate GFP concentration in planta and in protein extracts. These values were consistent with ELISA measurements of GFP in protein extracts from transgenic plants, indicating that the technique is a reliable measure of recombinant GFP expression. The levels of in planta GFP expression in both homozygous and hemizygous plants was then estimated. Homozygous transgenic plants expressed twice the amount of GFP than hemizygous plants, suggesting additive transgene expression. This methodology may be useful to simplify the characterization of transgene expression in plants.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.