ArticlePlanta2003
Localisation and characterisation of cell wall mannan polysaccharides in Arabidopsis thaliana.
Article in Planta, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 142 citations in OpenAlex.
- Atypical endo-β-1,4-mannanases are necessary for normal glucomannan synthesis in Arabidopsis.Plant physiology · 2026Article
- A Fresh Look at Celery Collenchyma and Parenchyma Cell Walls Through a Combination of Biochemical, Histochemical, and Transcriptomic Analyses.International journal of molecular sciences · 2025Article
- Convergent Emergence of Glucomannan β-Galactosyltransferase Activity in Asterids and Rosids.Plant & cell physiology · 2024Article
- Disruption of a DUF247 Containing Protein Alters Cell Wall Polysaccharides and Reduces Growth inPlants (Basel, Switzerland) · 2023Article
- Dynamics of cell wall polysaccharides during the elongation growth of rye primary roots.Planta · 2022Article
- Article
- ThePlant direct · 2021Article
- Multicolor 3D-dSTORM Reveals Native-State Ultrastructure of Polysaccharides' Network during Plant Cell Wall Assembly.iScience · 2020Article
- Multitarget Immunohistochemistry for Confocal and Super-resolution Imaging of Plant Cell Wall Polysaccharides.Bio-protocol · 2020Article
- Plant cell wall integrity maintenance in model plants and crop species-relevant cell wall components and underlying guiding principles.Cellular and molecular life sciences : CMLS · 2020Review
- Genome-wide identification of3 Biotech · 2020Article
- Mining MYB transcription factors from the genomes of orchids (Phalaenopsis and Dendrobium) and characterization of an orchid R2R3-MYB gene involved in water-soluble polysaccharide biosynthesis.Scientific reports · 2019Article
- The Patterned Structure of Galactoglucomannan Suggests It May Bind to Cellulose in Seed Mucilage.Plant physiology · 2018Article
- Exploring Cell Wall Composition and Modifications During the Development of the Gynoecium Medial Domain inFrontiers in plant science · 2018Article
- Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE.Journal of visualized experiments : JoVE · 2017Article
- Cytochemical Localization of Polysaccharides inFrontiers in plant science · 2017Article
- Arabidopsis CSLD5 Functions in Cell Plate Formation in a Cell Cycle-Dependent Manner.The Plant cell · 2016Article
- Regulation of Meristem Morphogenesis by Cell Wall Synthases in Arabidopsis.Current biology : CB · 2016Article
- Heteromannan and Heteroxylan Cell Wall Polysaccharides Display Different Dynamics During the Elongation and Secondary Cell Wall Deposition Phases of Cotton Fiber Cell Development.Plant & cell physiology · 2015Article
- MUCILAGE-RELATED10 Produces Galactoglucomannan That Maintains Pectin and Cellulose Architecture in Arabidopsis Seed Mucilage.Plant physiology · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polysaccharides containing beta-1,4-mannosyl residues (mannans) are abundant in the lignified secondary cell walls of gymnosperms, and are also found as major seed storage polysaccharides in some plants, such as legume species. Although they have been found in a variety of angiosperm tissues, little is known about their presence and tissue localisation in the model angiosperm, Arabidopsis thaliana (L.) Heynh. In this study, antibodies that specifically recognised mannans in competitive ELISA experiments were raised in rabbits. Using these antibodies, we showed that Golgi-rich vesicles derived from Arabidopsis callus were able to synthesise mannan polysaccharides in vitro. Immunofluorescence light microscopy and immunogold electron microscopy of Arabidopsis inflorescence stem sections revealed that the mannan polysaccharide epitopes were localised in the thickened secondary cell walls of xylem elements, xylem parenchyma and interfascicular fibres. Similarly, mannan epitopes were present in the xylem of the leaf vascular bundles. Surprisingly, the thickened epidermal cell walls of both leaves and stems also contained abundant mannan epitopes. Low levels were observed in most other cell types examined. Thus, mannans are widespread in Arabidopsis tissues, and may be of particular significance in both lignified and non-lignified thickened cell walls. Polysaccharide analysis using carbohydrate gel electrophoresis (PACE) of cell wall preparations digested with a specific mannanase showed that there is glucomannan in inflorescence stems. The findings show that Arabidopsis can be used as a model plant in studies of the synthesis and functions of mannans.
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