ArticleJournal of clinical microbiology2003
Rapid detection of Candida albicans in clinical blood samples by using a TaqMan-based PCR assay.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 2 syntheses or guidelines pooled it, 97 citations in OpenAlex.
- Effectiveness of Practices To Increase Timeliness of Providing Targeted Therapy for Inpatients with Bloodstream Infections: a Laboratory Medicine Best Practices Systematic Review and Meta-analysis.Clinical microbiology reviews · 2016Pooled it
- PCR diagnosis of invasive candidiasis: systematic review and meta-analysis.Journal of clinical microbiology · 2011Pooled it
- Quantitative real-time PCR in the diagnosis of opportunistic infections in immunocompromised patients: Diagnostic performance, clinical utility, and future directions.Infectious diseases & immunity · 2026Review
- Molecular Identification ofJournal of dentistry (Shiraz, Iran) · 2023Article
- Updates in Laboratory Identification of Invasive Fungal Infection in Neonates.Microorganisms · 2023Review
- Photo-Fenton and TiOMolecules (Basel, Switzerland) · 2023Article
- Multiplex size marker (YEAST PLEX) for rapid and accurate identification of pathogenic yeasts.Journal of clinical laboratory analysis · 2022Article
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- PCR-detectableOpen life sciences · 2020Article
- Establishment and Application of Multiple Cross Displacement Amplification Coupled With Lateral Flow Biosensor (MCDA-LFB) for Visual and Rapid Detection ofFrontiers in cellular and infection microbiology · 2019Article
- Multicenter Evaluation of the Accelerate PhenoTest BC Kit for Rapid Identification and Phenotypic Antimicrobial Susceptibility Testing Using Morphokinetic Cellular Analysis.Journal of clinical microbiology · 2018Article
- Rapid Detection of Candida albicans by Polymerase Spiral Reaction Assay in Clinical Blood Samples.Frontiers in microbiology · 2016Article
- The Role of PCR in the Diagnosis of Candida Vulvovaginitis-a New Gold Standard?Current infectious disease reports · 2015Article
- A real time PCR assay on blood for diagnosis of invasive candidiasis in immunocompromised patient.Current medical mycology · 2015Article
- Development and validation of a quantitative PCR assay using multiplexed hydrolysis probes for detection and quantification of Theileria orientalis isolates and differentiation of clinically relevant subtypes.Journal of clinical microbiology · 2015Article
- Emerging technologies for rapid identification of bloodstream pathogens.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2014Review
- Molecular and nonmolecular diagnostic methods for invasive fungal infections.Clinical microbiology reviews · 2014Review
- Multi-probe real-time PCR identification of four common Candida species in blood culture broth.Mycopathologia · 2014Article
- Review
- Quantification and optimization of Candida albicans DNA in blood samples using Real- Time PCR.Reports of biochemistry & molecular biology · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a rapid and reproducible PCR assay for quantitation of the Candida albicans ribosomal DNA (rDNA) in clinical blood samples based on the TaqMan principle (Applied Biosystems), in which a signal is generated by cleavage of a template-specific probe during amplification. We used two fluorogenic probes based on universal, fungus-specific primers, one for the detection of C. albicans species DNA and one for the detection of all Candida genus DNA. C. albicans blastoconidia mixed with whole blood in a titration experiment yielded a linear PCR signal over a range of 3 orders of magnitude. The TaqMan-based PCR assay for C. albicans exhibited a low limit of detection (5 CFU/ml of blood) and an excellent reproducibility (96 to 99%). While the C. albicans species-specific probe had 100% specificity for C. albicans, all Candida genus-specific probes cross-reacted with other organisms likely to coinfect patients with C. albicans infections. On the basis of these data, we determined the C. albicans loads with a species-specific probe from 122 blood samples from 61 hematology or oncology patients with clinically proven or suspected systemic Candida infections. Eleven positive samples exhibited a wide range of C. albicans loads, extending from 5 to 100,475 CFU/ml of blood. The sensitivity and specificity of the present assay were 100 and 97%, respectively, compared with the results of blood culture. These data indicate that the TaqMan-based PCR assay for quantitation of C. albicans with a species-specific probe provides an attractive alternative for the identification and quantitation of C. albicans rDNA in pure cultures and blood samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.