ArticleJournal of clinical microbiology2003
Detection and identification of ciprofloxacin-resistant Yersinia pestis by denaturing high-performance liquid chromatography.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed.
- A Survey of Antimicrobial Resistance Determinants in Category A Select Agents, Exempt Strains, and Near-Neighbor Species.International journal of molecular sciences · 2020Article
- Lovastatin protects against experimental plague in mice.PloS one · 2010Article
- Effective plague vaccination via oral delivery of plant cells expressing F1-V antigens in chloroplasts.Infection and immunity · 2008Article
- Early indicators of exposure to biological threat agents using host gene profiles in peripheral blood mononuclear cells.BMC infectious diseases · 2008Article
- Study of proinflammatory responses induced by Yersinia pestis in human monocytes using cDNA arrays.Genes and immunity · 2007Article
- Rapid genotyping of CTX-M extended-spectrum beta-lactamases by denaturing high-performance liquid chromatography.Antimicrobial agents and chemotherapy · 2007Article
- Denaturing high-performance liquid chromatography detection of ribosomal mutations conferring macrolide resistance in gram-positive cocci.Antimicrobial agents and chemotherapy · 2004Article
Corrections and comments
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Authors and funding
6 authors.
Funding
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Abstract
Denaturing high-performance liquid chromatography (DHPLC) has been used extensively to detect genetic variation. We used this method to detect and identify Yersinia pestis KIM5 ciprofloxacin-resistant isolates by analyzing the quinolone resistance-determining region (QRDR) of the gyrase A gene. Sequencing of the Y. pestis KIM5 strain gyrA QRDR from 55 ciprofloxacin-resistant isolates revealed five mutation types. We analyzed the gyrA QRDR by DHPLC to assess its ability to detect point mutations and to determine whether DHPLC peak profile analysis could be used as a molecular fingerprint. In addition to the five mutation types found in our ciprofloxacin-resistant isolates, several mutations in the QRDR were generated by site-directed mutagenesis and analyzed to further evaluate this method for the ability to detect QRDR mutations. Furthermore, a blind panel of 42 samples was analyzed by screening for two mutant types to evaluate the potential diagnostic value of this method. Our results showed that DHPLC is an efficient method for detecting mutations in genes that confer antibiotic resistance.
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