ArticleJournal of clinical microbiology2003
Real-time PCR-based system for simultaneous quantification of human papillomavirus types associated with high risk of cervical cancer.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Accuracy of real-time PCR assays for human papillomavirus using urine samples: a systematic review and meta-analysis.Journal of clinical microbiology · 2025Pooled it
- Randomised study shows that repeated self-sampling and HPV test has more than two-fold higher detection rate of women with CIN2+ histology than Pap smear cytology.British journal of cancer · 2018Trial
- Novel diagnostics for improved treatment of gynecological cancer.Upsala journal of medical sciences · 2025Review
- Detection of HPV DNA in Saliva of Patients with HPV-Associated Oropharyngeal Cancer Treated with Radiotherapy.Current oncology (Toronto, Ont.) · 2024Article
- History of HPV in HPV-positive elderly women.European journal of obstetrics & gynecology and reproductive biology: X · 2024Article
- Detection of high-risk Human Papillomavirus in prostate cancer from a UK based population.Scientific reports · 2023Article
- Prevalence of Human Papillomavirus in the Oropharynx of Healthy Individuals in an Italian Population.Journal of clinical medicine · 2022Article
- Dynamic Aqueous Multiphase Reaction System for One-Pot CRISPR-Cas12a-Based Ultrasensitive and Quantitative Molecular Diagnosis.Analytical chemistry · 2020Article
- HPV viral load in self-collected vaginal fluid samples as predictor for presence of cervical intraepithelial neoplasia.Virology journal · 2019Article
- Clinical validation of the HPVIR high-risk HPV test on cervical samples according to the international guidelines for human papillomavirus DNA test requirements for cervical cancer screening.Virology journal · 2019Article
- Synergistically enhanced colorimetric molecular detection using smart cup: a case for instrument-free HPV-associated cancer screening.Theranostics · 2019Article
- Nationwide comprehensive human papillomavirus (HPV) genotyping of invasive cervical cancer.British journal of cancer · 2018Article
- Type-specific high-risk human papillomavirus viral load as a viable triage indicator for high-grade squamous intraepithelial lesion: a nested case- control study.Cancer management and research · 2018Article
- Interactions Between High- and Low-Risk HPV Types Reduce the Risk of Squamous Cervical Cancer.Journal of the National Cancer Institute · 2015Article
- The DNA load of six high-risk human papillomavirus types and its association with cervical lesions.BMC cancer · 2015Article
- Persistence, clearance and reinfection regarding six high risk human papillomavirus types in Colombian women: a follow-up study.BMC infectious diseases · 2014Article
- HPV infection in esophageal squamous cell carcinoma and its relationship to the prognosis of patients in northern China.TheScientificWorldJournal · 2014Article
- Prognostic significance of high-risk human papillomavirus and p16(INK4A) in patients with esophageal squamous cell carcinoma.International journal of clinical and experimental medicine · 2014Article
- Results of cytology and high-risk human papillomavirus testing in females with cervical adenocarcinomaOncology letters · 2013Article
- Prevalence and type distribution of high-risk human papillomavirus in patients with cervical cancer: a population-based study.Infectious agents and cancer · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously shown that women with a high titer of human papillomavirus type 16 (HPV16) in cervical epithelial cells have an increased risk of developing cervical carcinoma in situ. In order to study the relationship between viral DNA amount and risk of cervical carcinoma for the HPV types most commonly found in cervical tumors, we developed a real-time PCR assay for the detection and quantification of HPV16, -18, -31, -33, -35, -39, -45, -52, -58, and -67. These HPV types are analyzed in two reaction tubes, allowing for independent quantification of three viral types, or groups of viral types, in each reaction. A separate reaction is used for estimating the number of a nuclear single-copy gene and is used to calculate the HPV copy number per genomic DNA equivalent in the sample. The system has a dynamic range from 10(2) to 10(7) HPV copies per assay and is applicable to both fresh clinical samples and DNA extracted from archival samples. Reconstitution experiments, made to mimic infections with several HPV types, shows that individual HPV types can be detected in a mixture as long as they represent 1 to 10% of the main type. The system was evaluated with respect to technical specificity and sensitivity, reproducibility, reagent stability, and sample preparation protocol and then used to analyze clinical samples. This homogeneous assay provides a fast and sensitive way for estimating the viral load of a series of the most frequent oncogenic HPV types in biopsies, as well as cervical smear samples.
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