ArticleJournal of clinical microbiology2003
Molecular typing of methicillin-resistant Staphylococcus aureus: can PCR replace pulsed-field gel electrophoresis?
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Antimicrobial efficacy of preoperative skin antisepsis and clonal relationship to postantiseptic skin-and-wound flora in patients undergoing clean orthopedic surgery.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Trial
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- Profiles of Sensitivity to Antibiotics and Heavy Metals in Strains ofAntibiotics (Basel, Switzerland) · 2025Article
- SaLTy: a novelMicrobial genomics · 2024Article
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- Evaluation of the genetic relatedness ofIranian journal of basic medical sciences · 2020Article
- Characterization of methicillin-resistant3 Biotech · 2020Article
- Phenotypic and Genomic Analyses of Burkholderia stabilis Clinical Contamination, Switzerland.Emerging infectious diseases · 2019Article
- Occurrence and characterisation of biofilms in drinking water systems of broiler houses.BMC microbiology · 2019Article
- Whole Genome Sequencing-Based Comparison of Food Isolates ofFrontiers in microbiology · 2019Article
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- DNA sequence-based re-assessment of archived Cronobacter sakazakii strains isolated from dairy products imported into China between 2005 and 2006.BMC genomics · 2018Article
- Genetic diversity ofIranian journal of microbiology · 2018Article
- Molecular typing of3 Biotech · 2018Article
- Methicillin-resistantInfection and drug resistance · 2018Review
- Direct repeat unitCanadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2017Article
- Case report: when two and ½ men go camping….BMC infectious diseases · 2017Article
- Molecular typing of nosocomialIranian journal of basic medical sciences · 2016Article
- Comparison of competitive exclusion with classical cleaning and disinfection on bacterial load in pig nursery units.BMC veterinary research · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pulsed-field gel electrophoresis (PFGE) is considered the "gold standard" for molecular typing of methicillin-resistant Staphylococcus aureus (MRSA). However, the method is time-consuming and expensive, and its discriminatory power may not be necessary in outbreak situations. We used a rapid multiplex PCR-based method with published primers and compared the results with those obtained by PFGE. A total of 75 clinical isolates were typed: 59 strains originated from our prospectively collected clinical strains and were epidemiologically unrelated; 16 strains came from an outbreak that was epidemiologically well defined in time and space. A primer mix of the spa gene, the coa gene, and the hypervariable region adjacent to mecA gene was used for multiplex PCR. Both PFGE and PCR clustered the 75 strains into 41 different genotypes. Concordance of the results was 100% for strains originating from the outbreak. Overall, both methods produced concordant results in 72% of cases. A total of 16% were clustered together by PFGE, but not by PCR and 12% were clustered together by PCR but not by PFGE, respectively. The turnaround time was only 8 h for PCR but 5 days for PFGE. This PCR-based method is excellent for rapid and inexpensive typing of MRSA in an outbreak setting, but the discriminatory power and reproducibility are still insufficient to replace PFGE in longitudinal studies in the endemic setting.
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