ArticleJournal of clinical microbiology2003
Evaluation of culture methods and a DNA probe-based PCR assay for detection of Campylobacter species in clinical specimens of feces.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed.
- Editorial:Frontiers in microbiology · 2022Article
- Article
- Intra-abdominal infection withAccess microbiology · 2021Article
- Article
- Isolation of emerging Campylobacter species in working farm dogs and their frozen home-killed raw meat diets.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Inquiring into the Gaps of Campylobacter Surveillance Methods.Veterinary sciences · 2017Review
- Global Epidemiology of Campylobacter Infection.Clinical microbiology reviews · 2015Review
- Potential involvement of Campylobacter curvus and Haemophilus parainfluenzae in preterm birth.BMJ case reports · 2014Article
- The role of environmental reservoirs in human campylobacteriosis.International journal of environmental research and public health · 2013Review
- Improved detection of bacterial pathogens in patients presenting with gastroenteritis by use of the EntericBio real-time Gastro Panel I assay.Journal of clinical microbiology · 2013Article
- Development and application of a real-time polymerase chain reaction method for Campylobacter jejuni detection.World journal of gastroenterology · 2013Article
- Genome Sequence of Campylobacter showae UNSWCD, Isolated from a Patient with Crohn's Disease.Genome announcements · 2013Article
- Molecular diagnosis of diarrhea: current status and future potential.Current infectious disease reports · 2012Article
- Campylobacter concisus - A new player in intestinal disease.Frontiers in cellular and infection microbiology · 2012Review
- Comparative analyses of Campylobacter concisus strains reveal the genome of the reference strain BAA-1457 is not representative of the species.Gut pathogens · 2011Article
- Defining the causes of diarrhea: novel approaches.Current opinion in infectious diseases · 2011Article
- Comparative analysis of cultural isolation and PCR based assay for detection of campylobacter jejuni in food and faecal samples.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2011Article
- Sequencing and validation of the genome of a Campylobacter concisus reveals intra-species diversity.PloS one · 2011Article
- Enteric campylobacteria and RNA viruses associated with healthy and diarrheic humans in the Chinook health region of southwestern Alberta, Canada.Journal of clinical microbiology · 2011Article
- Three-hour molecular detection of Campylobacter, Salmonella, Yersinia, and Shigella species in feces with accuracy as high as that of culture.Journal of clinical microbiology · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Campylobacter species are the leading agents of bacterial gastroenteritis in developed countries. In this study 320 specimens of feces from patients with symptoms of acute gastroenteritis were cultured for Campylobacter species by direct plating on modified charcoal cefoperazone deoxycholate agar and by enrichment in modified Preston broth, with or without blood added, for 48 h at 37 degrees C prior to plating. A 16S/23S PCR/DNA probe membrane-based colorimetric detection assay was evaluated on a subset of the feces (n = 127), including 18 culture-positive and 109 culture-negative specimens. DNA was extracted directly from the fecal specimens by using the QIAamp DNA stool Minikit for the DNA probe-based PCR assay (PCR/DNA probe assay). A second PCR/DNA probe assay based on the 16S rRNA gene in Campylobacter spp. was applied to all specimens that were culture negative, PCR/DNA positive on initial analysis. Campylobacter species were cultured in 20 of the 320 specimens. The 16S/23S PCR/DNA probe assay detected campylobacter DNA in 17 of 18 (94% sensitivity) culture-positive specimens and in 41 (38%) culture-negative specimens. The presence of campylobacter DNA in 35 of 41 culture-negative specimens was confirmed by the 16S PCR/DNA probe assay. DNA sequence analysis of seven 16S/23S PCR products and five 16S PCR products amplified from a selection of these specimens confirmed the presence of campylobacter DNA and more specifically Campylobacter jejuni, C. concisus, C. curvus, and C. gracilis DNA in these specimens. The molecular assays described in this study are rapid methods for the detection and identification of Campylobacter species in fecal specimens. The finding of Campylobacter spp. DNA in a large number of specimens of feces from patients with no other identified cause of diarrhea may suggest that Campylobacter spp. other than C. jejuni and C. coli may account for a proportion of cases of acute gastroenteritis in which no etiological agent is currently identified.
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