ArticleJournal of clinical microbiology2003
Genome-wide DNA microarray analysis of Francisella tularensis strains demonstrates extensive genetic conservation within the species but identifies regions that are unique to the highly virulent F. tularensis subsp. tularensis.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers, 1 of them a synthesis that pooled it.
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Who cites it
94 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Large-scale comparative genomics meta-analysis of Campylobacter jejuni isolates reveals low level of genome plasticity.Journal of clinical microbiology · 2004Pooled it
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- Ulceroglandular form of tularemia after squirrel bite: a case report.Journal of medical case reports · 2022Article
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- Outbreak of Tularemia in a Group of Hunters in Germany in 2018-Kinetics of Antibody and Cytokine Responses.Microorganisms · 2020Article
- Optimized MALDI TOF Mass Spectrometry Identification ofMicroorganisms · 2020Article
- Molecular identification of the source of an uncommon tularaemia outbreak, Germany, autumn 2016.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2019Article
- Genetic Diversity and Spatial Segregation ofFrontiers in cellular and infection microbiology · 2019Article
- Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications.Sensors and actuators. B, Chemical · 2018Review
- First Draft Genome Sequences of Three Strains ofGenome announcements · 2017Article
- A novel broth medium for enhanced growth of Francisella tularensis.Letters in applied microbiology · 2017Article
- Russian isolates enlarge the known geographic diversity of Francisella tularensis subsp. mediasiatica.PloS one · 2017Article
- High and novel genetic diversity of Francisella tularensis in Germany and indication of environmental persistence.Epidemiology and infection · 2016Article
- Natural Selection in Virulence Genes of Francisella tularensis.Journal of molecular evolution · 2016Article
- The use of Matrix-assisted laser desorption ionization-time of flight mass spectrometry in the identification of Francisella tularensis.Bosnian journal of basic medical sciences · 2016Article
- Article
- Respiratory Tularemia:The open microbiology journal · 2016Article
34 more citing papers are in PubMed but not listed here.
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10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Francisella tularensis is a potent pathogen and a possible bioterrorism agent. Little is known, however, to explain the molecular basis for its virulence and the distinct differences in virulence found between the four recognized subspecies, F. tularensis subsp. tularensis, F. tularensis subsp. mediasiatica, F. tularensis subsp. holarctica, and F. tularensis subsp. novicida. We developed a DNA microarray based on 1,832 clones from a shotgun library used for sequencing of the highly virulent strain F. tularensis subsp. tularensis Schu S4. This allowed a genome-wide analysis of 27 strains representing all four subspecies. Overall, the microarray analysis confirmed a limited genetic variation within the species F. tularensis, and when the strains were compared, at most 3.7% of the probes showed differential hybridization. Cluster analysis of the hybridization data revealed that the causative agents of type A and type B tularemia, i.e., F. tularensis subsp. tularensis and F. tularensis subsp. holarctica, respectively, formed distinct clusters. Despite marked differences in their virulence and geographical origin, a high degree of genomic similarity between strains of F. tularensis subsp. tularensis and F. tularensis subsp. mediasiatica was apparent. Strains from Japan clustered separately, as did strains of F. tularensis subsp. novicida. Eight regions of difference (RD) 0.6 to 11.5 kb in size, altogether comprising 21 open reading frames, were identified that distinguished strains of the moderately virulent subspecies F. tularensis subsp. holarctica and the highly virulent subspecies F. tularensis subsp. tularensis. One of these regions, RD1, allowed for the first time the development of an F. tularensis-specific PCR assay that discriminates each of the four subspecies.
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