ArticleMolecular and cellular biochemistry2003
Regulation of cytosolic phospholipase A2, cyclooxygenase-1 and -2 expression by PMA, TNFalpha, LPS and M-CSF in human monocytes and macrophages.
Article in Molecular and cellular biochemistry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 47 citations in OpenAlex.
- Low molecular weight hyaluronan activates cytosolic phospholipase A2α and eicosanoid production in monocytes and macrophages.The Journal of biological chemistry · 2014Trial
- Early pregnancy loss in 15-hydroxyprostaglandin dehydrogenase knockout (15-HPGDScientific reports · 2019Article
- Immune modulation to improve tissue engineering outcomes for cartilage repair in the osteoarthritic joint.Tissue engineering. Part B, Reviews · 2015Review
- The step further to understand the role of cytosolic phospholipase A2 alpha and group X secretory phospholipase A2 in allergic inflammation: pilot study.BioMed research international · 2014Article
- The involvement of phospholipases A2 in asthma and chronic obstructive pulmonary disease.Mediators of inflammation · 2013Review
- Study on the relationship of cPLA2, CK-MB, and membrane phospholipid content in acute myocardial infarction.Heart and vessels · 2011Article
- Characterization of proteins associating with 5' terminus of PGHS-1 mRNA.Cellular & molecular biology letters · 2010Article
- Global transcriptional response of macrophage-like THP-1 cells to Shiga toxin type 1.Infection and immunity · 2010Article
- TLR-4 mediated group IVA phospholipase A(2) activation is phosphatidic acid phosphohydrolase 1 and protein kinase C dependent.Biochimica et biophysica acta · 2009Article
- Activation of cytosolic phospholipase A2-{alpha} as a novel mechanism regulating endothelial cell cycle progression and angiogenesis.The Journal of biological chemistry · 2009Article
- Expression of phospholipases A2 in primary human lung macrophages: role of cytosolic phospholipase A2-alpha in arachidonic acid release and platelet activating factor synthesis.Biochimica et biophysica acta · 2009Article
- Phosphatidic acid phosphohydrolase in the regulation of inflammatory signaling.Advances in enzyme regulation · 2009Review
- Sumoylation of LAP1 is involved in the HDAC4-mediated repression of COX-2 transcription.Nucleic acids research · 2008Article
- 17-Beta estradiol enhances prostaglandin E2 production in human U937-derived macrophages.Molecular and cellular biochemistry · 2004Article
- Expression of cPLAInternational journal of immunopathology and pharmacologyArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytosolic phospholipases A2 (cPLA2) and cyclooxygenases-1 and -2 (COX-1 and -2) play a pivotal role in the metabolism of arachidonic acid (AA) and in eicosanoid production. The coordinate regulation and expression of these enzymes is not well defined. In this study, the effect of phorbol 12-myristate 13-acetate (PMA), tumor necrosis factor alpha (TNFalpha), lipopolysaccharide (LPS) and macrophage-colony stimulating factor (M-CSF) on AA release and prostaglandin E2 (PGE2) production and the expression of cPLA2 and COX-1 and -2 were investigated in U937 human pre-monocytic cells and fully differentiated macrophages. Treatment of U937 cells with PMA or macrophages with LPS increased AA release and PGE2 production. Incubation of U937 cells or macrophages for 8 h with all stimuli elevated cPLA2 expression. In contrast, cPLA2 expression was reduced upon further incubation of U937 cells or macrophages for 24 h with all stimuli indicating a bi-phasic expression pattern of this enzyme. PMA induced COX-1 expression in U937 cells whereas LPS induced COX-2 expression in macrophages. Although TNFalpha and M-CSF induced a significant amount of AA release in both cell models, they failed to induce a comparable production of PGE2 since they were unable to induce the coordinate expression of the downstream key enzymes, COX-1 or COX-2. The results suggest that the enhancement of AA release in both U937 cells and macrophages may be caused by both increased cPLA2 activity and elevated cPLA2 protein expression. In addition, PMA stimulates PGE2 production via up-regulation of COX-1, and likely COX-2, expression in U937 cells whereas LPS stimulates PGE2 production via induction of COX-2 expression in macrophages.
Indexed as
Identifiers
12841340W1499274698What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.