ArticleThe Journal of clinical investigation2003
Luminal NaCl delivery regulates basolateral PGE2 release from macula densa cells.
Article in The Journal of clinical investigation, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed, 127 citations in OpenAlex.
- Degree of Cyclooxygenase-2 Inhibition Modulates Blood Pressure Response.Hypertension (Dallas, Tex. : 1979) · 2026Trial
- Hold the salt for kidney regeneration.The Journal of clinical investigation · 2024Article
- NaHypertension (Dallas, Tex. : 1979) · 2022Article
- A new view of macula densa cell protein synthesis.American journal of physiology. Renal physiology · 2021Article
- A new view of macula densa cell microanatomy.American journal of physiology. Renal physiology · 2021Article
- Low Salt Delivery Triggers Autocrine Release of Prostaglandin E2 From the Aldosterone-Sensitive Distal Nephron in Familial Hyperkalemic Hypertension Mice.Frontiers in physiology · 2021Article
- Hydrochlorothiazide ameliorates polyuria caused by tolvaptan treatment of polycystic kidney disease in PCK rats.Clinical and experimental nephrology · 2019Article
- "I don't get no respect": the role of chloride in acute kidney injury.American journal of physiology. Renal physiology · 2019Review
- The macula densa prorenin receptor is essential in renin release and blood pressure control.American journal of physiology. Renal physiology · 2018Article
- Phenotypic dissection of the mouseThe Journal of biological chemistry · 2018Article
- Interactions between the Cyclooxygenase Metabolic Pathway and the Renin-Angiotensin-Aldosterone Systems: Their Effect on Cardiovascular Risk, from Theory to the Clinical Practice.BioMed research international · 2018Review
- PGEAmerican journal of physiology. Renal physiology · 2017Article
- Prostaglandin EThe American journal of the medical sciences · 2017Article
- Endocrine functions of the renal interstitium.Pflugers Archiv : European journal of physiology · 2017Review
- Effect of Cyclooxygenase(COX)-1 and COX-2 inhibition on furosemide-induced renal responses and isoform immunolocalization in the healthy cat kidney.BMC veterinary research · 2015Article
- Chloride secretion by renal collecting ducts.Current opinion in nephrology and hypertension · 2015Article
- Newly stemming functions of macula densa-derived prostanoids.Hypertension (Dallas, Tex. : 1979) · 2015Article
- Salt restriction leads to activation of adult renal mesenchymal stromal cell-like cells via prostaglandin E2 and E-prostanoid receptor 4.Hypertension (Dallas, Tex. : 1979) · 2015Article
- The hidden hand of chloride in hypertension.Pflugers Archiv : European journal of physiology · 2015Review
- Salt feedback on the renin-angiotensin-aldosterone system.Pflugers Archiv : European journal of physiology · 2015Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Macula densa (MD) cells express COX-2 and COX-2-derived PGs appear to signal the release of renin from the renal juxtaglomerular apparatus, especially during volume depletion. However, the synthetic machinery and identity of the specific prostanoid released from intact MD cells remains uncertain. In the present studies, a novel biosensor tool was engineered to directly determine whether MD cells release PGE2 in response to low luminal NaCl concentration ([NaCl]L). HEK293 cells were transfected with the Ca2+-coupled E-prostanoid receptor EP1 (HEK/EP1) and loaded with fura-2. HEK/EP1 cells produced a significant elevation in intracellular [Ca2+] ([Ca2+]i) by 29.6 +/- 12.8 nM (n = 6) when positioned at the basolateral surface of isolated perfused MD cells and [NaCl]L was reduced from 150 mM to zero. HEK/EP1 [Ca2+]i responses were observed mainly in preparations from rabbits on a low-salt diet and were completely inhibited by either a selective COX-2 inhibitor or an EP1 antagonist, and also by 100 microM luminal furosemide. Also, 20-mM graduated reductions in [NaCl]L between 80 and 0 mM caused step-by-step increases in HEK/EP1 [Ca2+]i. Low-salt diet greatly increased the expression of both COX-2 and microsome-associated PGE synthase (mPGES) in the MD. These studies provide the first direct evidence that intact MD cells synthesize and release PGE2 during reduced luminal salt content and suggest that this response is important in the control of renin release and renal vascular resistance during salt deprivation.
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Registered trials
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