ArticleGenome research2003
Identification of novel ErbB3-interacting factors using the split-ubiquitin membrane yeast two-hybrid system.
Article in Genome research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 120 citations in OpenAlex.
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- Yeast Two-Hybrid Assay for Investigating Antiviral Innate Immunity.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Cell-Int: a cell-cell interaction assay to identify native membrane protein interactions.Life science alliance · 2024Article
- Protein P5 of pear chlorotic leaf spot-associated virus is a pathogenic factor that suppresses RNA silencing and enhances virus movement.Molecular plant pathology · 2024Article
- RGS4 controls airway hyperresponsiveness through GAP-independent mechanisms.The Journal of biological chemistry · 2024Article
- Detection of Protein-Protein Interactions Utilizing the Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The KFrontiers in plant science · 2023Article
- NbTMP14 Is Involved in Tomato Spotted Wilt Virus Infection and Symptom Development by Interaction with the Viral NSm Protein.Viruses · 2021Article
- Identification and screening of host proteins interacting with ORFV-ORF047 protein.Virology journal · 2021Article
- Phosphorylation of a malate transporter promotes malate excretion and reduces cadmium uptake in apple.Journal of experimental botany · 2020Article
- The Potato Virus X TGBp2 Protein Plays Dual Functional Roles in Viral Replication and Movement.Journal of virology · 2019Article
- An apple sucrose transporter MdSUT2.2 is a phosphorylation target for protein kinase MdCIPK22 in response to drought.Plant biotechnology journal · 2019Article
- The Glucose Sensor MdHXK1 Phosphorylates a Tonoplast NaPlant physiology · 2018Article
- A Global Analysis of the Receptor Tyrosine Kinase-Protein Phosphatase Interactome.Molecular cell · 2017Article
- Development of a membrane-anchored ligand and receptor yeast two-hybrid system for ligand-receptor interaction identification.Scientific reports · 2016Article
- Tryptophan-2,3-dioxygenase (TDO) inhibition ameliorates neurodegeneration by modulation of kynurenine pathway metabolites.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Tyrosine Phosphorylation Based Homo-dimerization of Arabidopsis RACK1A Proteins Regulates Oxidative Stress Signaling Pathways in Yeast.Frontiers in plant science · 2016Article
- Effect of C-Terminal S-Palmitoylation on D2 Dopamine Receptor Trafficking and Stability.PloS one · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analysis of membrane protein interactions is difficult because of the hydrophobic nature of these proteins, which often renders conventional biochemical and genetic assays fruitless. This is a substantial problem because proteins that are integral or associated with membranes represent approximately one-third of all proteins in a typical eukaryotic cell. We have shown previously that the modified split-ubiquitin system can be used as a genetic assay for the in vivo detection of interactions between the two characterized yeast transmembrane proteins, Ost1p and Wbp1p. This so-called split-ubiquitin membrane yeast two-hybrid (YTH) system uses the split-ubiquitin approach in which reconstitution of two ubiquitin halves is mediated by a protein-protein interaction. Here we converted the split-ubiquitin membrane YTH system into a generally applicable in vivo screening approach to identify interacting partners of a particular mammalian transmembrane protein. We have demonstrated the effectiveness of this approach by using the mammalian ErbB3 receptor as bait and have identified three previously unknown ErbB3-interacting proteins. In addition, we have confirmed one of the newly found interactions between ErbB3 and the membrane-associated RGS4 protein by coimmunoprecipitating the two proteins from human cells. We expect the split-ubiquitin membrane YTH technology to be valuable for the identification of potential interacting partners of integral membrane proteins from many model organisms.
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