ArticleApplied and environmental microbiology2003
Identification of two-component regulatory systems in Bifidobacterium infantis by functional complementation and degenerate PCR approaches.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 18 citations in OpenAlex.
- A two-component regulatory system controls autoregulated serpin expression in Bifidobacterium breve UCC2003.Applied and environmental microbiology · 2012Article
- A conserved two-component signal transduction system controls the response to phosphate starvation in Bifidobacterium breve UCC2003.Applied and environmental microbiology · 2012Article
- A bile-inducible membrane protein mediates bifidobacterial bile resistance.Microbial biotechnology · 2012Article
- Discovering novel bile protection systems in Bifidobacterium breve UCC2003 through functional genomics.Applied and environmental microbiology · 2012Article
- Cloning and characterization of the bile salt hydrolase genes (bsh) from Bifidobacterium bifidum strains.Applied and environmental microbiology · 2004Article
- Characterization and heterologous expression of the oxalyl coenzyme A decarboxylase gene from Bifidobacterium lactis.Applied and environmental microbiology · 2004Article
- Bifidobacterium lactis DSM 10140: identification of the atp (atpBEFHAGDC) operon and analysis of its genetic structure, characteristics, and phylogeny.Applied and environmental microbiology · 2004Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two-component signal transduction systems (2CSs) are widely used by bacteria to sense and adapt to changing environmental conditions. With two separate approaches, three different 2CSs were identified on the chromosome of the probiotic bacterium Bifidobacterium infantis UCC 35624. One locus was identified by means of functional complementation of an Escherichia coli mutant. Another two were identified by PCR with degenerate primers corresponding to conserved regions of one protein component of the 2CS. The complete coding regions for each gene cluster were obtained, which showed that each 2CS-encoding locus specified a histidine protein kinase and an assumed cognate response regulator. Transcriptional analysis of the 2CSs by Northern blotting and primer extension identified a number of putative promoter sequences for this organism while revealing that the expression of each 2CS was growth phase dependent. Analysis of the genetic elements involved revealed significant homology with several distinct regulatory families from other high-G+C-content bacteria. The conservation of the genetic organization of these three 2CSs in other bacteria, including a number of recently published Bifidobacterium genomes, was investigated.
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Registered trials
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