ArticleApplied and environmental microbiology2003
Development of reverse transcription (RT)-PCR and real-time RT-PCR assays for rapid detection and quantification of viable yeasts and molds contaminating yogurts and pasteurized food products.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 185 citations in OpenAlex.
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- Effects of age on lacrimal gland bioactive lipids.The ocular surface · 2024Article
- Advances in detection methods for viable Salmonella spp.: current applications and challenges.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Review
- Deep learning-based approach for detecting COVID-19 in chest X-rays.Biomedical signal processing and control · 2022Article
- The Effects of Polyphenol, Tannic Acid, or Tannic Acid in Combination with Pamidronate on Human Osteoblast Cell Line Metabolism.Molecules (Basel, Switzerland) · 2022Article
- Transcriptome Analysis of Viable but Non-CulturableFrontiers in microbiology · 2022Article
- Stool preparation under anaerobic conditions contributes to retention of obligate anaerobes: potential improvement for fecal microbiota transplantation.BMC microbiology · 2021Article
- Plasmonically Enhanced CRISPR/Cas13a-Based Bioassay for Amplification-Free Detection of Cancer-Associated RNA.Advanced healthcare materials · 2021Article
- Trends and Applications of Omics Technologies to Functional Characterisation of Enzymes and Protein Metabolites Produced by Fungi.Journal of fungi (Basel, Switzerland) · 2021Review
- Article
- Analysis of methods for quantifying yeast cell concentration in complex lignocellulosic fermentation processes.Scientific reports · 2021Article
- Chest X-ray image phase features for improved diagnosis of COVID-19 using convolutional neural network.International journal of computer assisted radiology and surgery · 2021Article
- Microbiological and Chemical Characteristics of Wet Coffee Fermentation Inoculated WithFrontiers in microbiology · 2021Article
- Austrian Raw-Milk Hard-Cheese Ripening Involves Successional Dynamics of Non-Inoculated Bacteria and Fungi.Foods (Basel, Switzerland) · 2020Article
- Gaps in the assortment of rapid assays for microorganisms of interest to the dairy industry.Advances in applied microbiology · 2020Review
- Molecular assays to detect the presence and viability of Phytophthora ramorum and Grosmannia clavigera.PloS one · 2020Article
- High-Throughput Rapid and Inexpensive Assay for Quantitative Determination of Low Cell-Density Yeast Cultures.Microorganisms · 2019Article
- HCV Detection, Discrimination, and Genotyping Technologies.Sensors (Basel, Switzerland) · 2018Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse transcriptase PCR (RT-PCR) and real-time RT-PCR assays have been used to detect and quantify actin mRNA from yeasts and molds. Universal primers were designed based on the available fungal actin sequences, and by RT-PCR they amplified a specific 353-bp fragment from fungal species involved in food spoilage. From experiments on heat-treated cells, actin mRNA was a good indicator of cell viability: viable cells and cells in a nonculturable state were detected, while no signal was observed from dead cells. The optimized RT-PCR assay was able to detect 10 CFU of fungi ml(-1) in pure culture and 10(3) and 10(2) CFU ml(-1) in artificially contaminated yogurts and pasteurized fruit-derived products, respectively. Real-time RT-PCR, performed on a range of spoiled commercial food products, validated the suitability of actin mRNA detection for the quantification of naturally contaminating fungi. The specificity and sensitivity of the procedure, combined with its speed, its reliability, and the potential automation of the technique, offer several advantages to routine analysis programs that assess the presence and viability of fungi in food commodities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.