ArticleApplied and environmental microbiology2003
Characterization of genes involved in the metabolism of alpha-galactosides by Lactococcus raffinolactis.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 52 citations in OpenAlex.
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- Effect of Lactic Acid Bacteria on the Lipid Profile of Bean-Based Plant Substitute of Fermented Milk.Microorganisms · 2020Article
- Characterization of a potential probiotic bacterium Lactococcus raffinolactis WiKim0068 isolated from fermented vegetable using genomic and in vitro analyses.BMC microbiology · 2020Article
- Profiling of koumiss microbiota and organic acids and their effects on koumiss taste.BMC microbiology · 2020Article
- GlaR (YugA)-a novel RpiR-family transcription activator of the Leloir pathway of galactose utilization in Lactococcus lactis IL1403.MicrobiologyOpen · 2019Article
- Assessment of the microbial diversity of Chinese Tianshan tibicos by single molecule, real-time sequencing technology.Food science and biotechnology · 2019Article
- From Genome to Phenotype: An Integrative Approach to Evaluate the Biodiversity of Lactococcus lactis.Microorganisms · 2017Review
- ClaR--a novel key regulator of cellobiose and lactose metabolism in Lactococcus lactis IL1403.Applied microbiology and biotechnology · 2015Article
- The carbohydrate metabolism signature of lactococcus lactis strain A12 reveals its sourdough ecosystem origin.Applied and environmental microbiology · 2013Article
- Genome-scale genotype-phenotype matching of two Lactococcus lactis isolates from plants identifies mechanisms of adaptation to the plant niche.Applied and environmental microbiology · 2008Article
- A thermostable alpha-galactosidase from Lactobacillus fermentum CRL722: genetic characterization and main properties.Current microbiology · 2006Article
- Global analysis of carbohydrate utilization by Lactobacillus acidophilus using cDNA microarrays.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Expression and site-directed mutagenesis of the lactococcal abortive phage infection protein AbiK.Journal of bacteriology · 2005Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactococcus raffinolactis, unlike most lactococci, is able to ferment alpha-galactosides, such as melibiose and raffinose. More than 12 kb of chromosomal DNA from L. raffinolactis ATCC 43920 was sequenced, including the alpha-galactosidase gene and genes involved in the Leloir pathway of galactose metabolism. These genes are organized into an operon containing aga (alpha-galactosidase), galK (galactokinase), and galT (galactose 1-phosphate uridylyltransferase). Northern blotting experiments revealed that this operon was induced by galactosides, such as lactose, melibiose, raffinose, and, to a lesser extent, galactose. Similarly, alpha-galactosidase activity was higher in lactose-, melibiose-, and raffinose-grown cells than in galactose-grown cells. No alpha-galactosidase activity was detected in glucose-grown cells. The expression of the aga-galKT operon was modulated by a regulator encoded by the upstream gene galR. The product of galR belongs to the LacI/GalR family of transcriptional regulators. In L. lactis, L. raffinolactis GalR acted as a repressor of aga and lowered the enzyme activity by more than 20-fold. We suggest that the expression of the aga operon in lactococci is negatively controlled by GalR and induced by a metabolite derived from the metabolism of galactosides.
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