ArticleApplied and environmental microbiology2003
Pilin-like proteins in the extremely thermophilic bacterium Thermus thermophilus HB27: implication in competence for natural transformation and links to type IV pilus biogenesis.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Structural insights into the Thermus thermophilus type IV pilus machinery assembling two distinct pili.Communications biology · 2026Article
- Review
- Phylogenomic analyses and comparative genomic studies of Thermus strains isolated from Tengchong and Tibet Hot Springs, China.Antonie van Leeuwenhoek · 2024Article
- Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation.Extremophiles : life under extreme conditions · 2021Review
- Function and Benefits of Natural Competence in Cyanobacteria: From Ecology to Targeted Manipulation.Life (Basel, Switzerland) · 2020Review
- Cryo-electron microscopy reveals two distinct type IV pili assembled by the same bacterium.Nature communications · 2020Article
- Article
- The traffic ATPase PilF interacts with the inner membrane platform of the DNA translocator and type IV pili fromFEBS open bio · 2019Article
- DNA Uptake by Type IV Filaments.Frontiers in molecular biosciences · 2019Review
- Functional dissection of the three N-terminal general secretory pathway domains and the Walker motifs of the traffic ATPase PilF from Thermus thermophilus.Extremophiles : life under extreme conditions · 2018Article
- The transjugation machinery of Thermus thermophilus: Identification of TdtA, an ATPase involved in DNA donation.PLoS genetics · 2017Article
- Structures of type IV pilins from Thermus thermophilus demonstrate similarities with type II secretion system pseudopilins.Journal of structural biology · 2016Article
- Whole Genome Comparison of Thermus sp. NMX2.A1 Reveals Principle Carbon Metabolism Differences with Closest Relation Thermus scotoductus SA-01.G3 (Bethesda, Md.) · 2016Article
- Topology and Structure/Function Correlation of Ring- and Gate-forming Domains in the Dynamic Secretin Complex of Thermus thermophilus.The Journal of biological chemistry · 2016Article
- Article
- A mutant ('lab strain') of the hyperthermophilic archaeon Pyrococcus furiosus, lacking flagella, has unusual growth physiology.Extremophiles : life under extreme conditions · 2015Article
- Noncanonical cell-to-cell DNA transfer in Thermus spp. is insensitive to argonaute-mediated interference.Journal of bacteriology · 2015Article
- Zinc and ATP binding of the hexameric AAA-ATPase PilF from Thermus thermophilus: role in complex stability, piliation, adhesion, twitching motility, and natural transformation.The Journal of biological chemistry · 2014Article
- Analysis of genomic rearrangements, horizontal gene transfer and role of plasmids in the evolution of industrial important Thermus species.BMC genomics · 2014Article
- Type IV pilus biogenesis, twitching motility, and DNA uptake in Thermus thermophilus: discrete roles of antagonistic ATPases PilF, PilT1, and PilT2.Applied and environmental microbiology · 2014Article
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4 authors.
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Abstract
The extreme thermophile Thermus thermophilus HB27 exhibits high frequencies of natural transformation. Although we recently reported identification of the first competence genes in Thermus, the molecular basis of DNA uptake is unknown. A pilus-like structure is assumed to be involved. Twelve genes encoding prepilin-like proteins were identified in three loci in the genome of T. thermophilus. Mutational analyses, described in this paper, revealed that one locus, which contains four genes that encode prepilin-like proteins (pilA1 to pilA4), is essential for natural transformation. Additionally, comZ, a new competence gene with no similarity to known genes, was identified. Analysis of the piliation phenotype revealed wild-type piliation of a pilA1-pilA3Deltakat mutant and a comZ mutant, whereas a pilA4 mutant was found to be completely devoid of pilus structures. These findings, together with the significant similarity of PilA4 to prepilins, led to the conclusion that the T. thermophilus pilus structures are type IV pili. Furthermore, the loss of the transformation and piliation phenotype in the pilA4 mutant suggests that type IV pili are implicated in natural transformation of T. thermophilus HB27.
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