ArticleHistochemistry and cell biology2003
Expression of cathepsin H in differentiating rat spermatids: immunoelectron microscopic study.
Article in Histochemistry and cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 14 citations in OpenAlex.
- Understanding the Underlying Molecular Mechanisms of Meiotic Arrest during In Vitro Spermatogenesis in Rat Prepubertal Testicular Tissue.International journal of molecular sciences · 2022Article
- Nuage proteins: their localization in subcellular structures of spermatogenic cells as revealed by immunoelectron microscopy.Histochemistry and cell biology · 2012Review
- Ubiquitin signals in the developing acrosome during spermatogenesis of rat testis: an immunoelectron microscopic study.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2004Article
- Innovative techniques and applications in histochemistry and cell biology.Histochemistry and cell biology · 2003Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of cathepsin H (CH) in differentiating rat spermatids was studied by an immunoelectron microscopic technique. Cathepsin H was detected in the acrosome throughout differentiation steps but cathepsins B, D, and L and lysosomal membrane protein (LGP107) were not. Early in the formation of the acrosome, CH signals were observed in Golgi vesicles but not in acrosomal vesicles. At steps 3-4, CH signals were associated with a fibrous material attached to the inner surface of the vesicle membrane on the Golgi side. At steps 5-6, this fibrous material accumulated to form an electron-dense sheet to which CH signals were confined. The rest of the acrosome was negative for the enzyme. At steps 11-12, the CH-positive fibrous sheet expanded from the apical to the ventral side of the sperm head. After step 16, the surface of outer dense fibers in the flagellar axoneme and reticulated bodies were stained for CH. In epididymal sperm, CH signals were detected in the acrosome as well as on the surface of the outer dense fibers running from the middle to the principal piece. By immunofluorescence staining, CH was found to be localized to the acrosome, middle piece, and principal piece.
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Registered trials
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