ArticleThe Plant cell2003
High-throughput viral expression of cDNA-green fluorescent protein fusions reveals novel subcellular addresses and identifies unique proteins that interact with plasmodesmata.
Article in The Plant cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
59 citing papers in PubMed, 205 citations in OpenAlex.
- The RALF-FERONIA Signaling Axis: A Central Hub Integrating Plant Growth, Reproduction, and Stress Responses.International journal of molecular sciences · 2025Review
- Pull the fuzes: Processing protein precursors to generate apoplastic danger signals for triggering plant immunity.Plant communications · 2024Review
- The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize.The Plant cell · 2024Article
- High-throughput investigation of genetic design constraints in domesticated Influenza A Virus for transient gene delivery.bioRxiv : the preprint server for biology · 2024Article
- Lateral Root Initiation in Cucumber (International journal of molecular sciences · 2023Article
- Salivary carbonic anhydrase II in winged aphid morph facilitates plant infection by viruses.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Membrane contact sites and cytoskeleton-membrane interactions in autophagy.FEBS letters · 2022Review
- Vacuolar membrane structures and their roles in plant-pathogen interactions.Plant molecular biology · 2019Review
- Development of a new tobamovirus-based viral vector for protein expression in plants.Molecular biology reports · 2019Article
- Article
- Proteomic Analysis of Plasmodesmata FromFrontiers in plant science · 2018Article
- KinG Is a Plant-Specific Kinesin That Regulates Both Intra- and Intercellular Movement of SHORT-ROOT.Plant physiology · 2018Article
- BAK1 is involved in AtRALF1-induced inhibition of root cell expansion.PLoS genetics · 2017Article
- Overexpression ofFrontiers in plant science · 2017Article
- Low-Phosphate Induction of Plastidal Stromules Is Dependent on Strigolactones But Not on the Canonical Strigolactone Signaling Component MAX2.Plant physiology · 2016Article
- Review
- Article
- REGULATOR OF BULB BIOGENESIS1 (RBB1) Is Involved in Vacuole Bulb Formation in Arabidopsis.PloS one · 2015Article
- Dynamics of vacuoles and H+-pyrophosphatase visualized by monomeric green fluorescent protein in Arabidopsis: artifactual bulbs and native intravacuolar spherical structures.The Plant cell · 2014Article
- Arabidopsis thaliana RALF1 opposes brassinosteroid effects on root cell elongation and lateral root formation.Journal of experimental botany · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A strategy was developed for the high-throughput localization of unknown expressed proteins in Nicotiana benthamiana. Libraries of random, partial cDNAs fused to the 5' or 3' end of the gene for green fluorescent protein (GFP) were expressed in planta using a vector based on Tobacco mosaic virus. Viral populations were screened en masse on inoculated leaves using a confocal microscope fitted with water-dipping lenses. Each viral infection site expressed a unique cDNA-GFP fusion, allowing several hundred cDNA-GFP fusions to be screened in a single day. More than half of the members of the library carrying cDNA fusions to the 5' end of gfp that expressed fluorescent fusion proteins displayed discrete, noncytosolic, subcellular localizations. Nucleotide sequence determination of recovered cDNA sequences and subsequent sequence searches showed that fusions of GFP to proteins that had a predicted subcellular "address" became localized with high fidelity. In a subsequent screen of >20,000 infection foci, 12 fusion proteins were identified that localized to plasmodesmata, a subcellular structure for which very few protein components have been identified. This virus-based system represents a method for high-throughput functional genomic study of plant cell organelles and allows the identification of unique proteins that associate with specific subcompartments within organelles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.