ArticlePlant cell reports2003
Production of Hevea brasiliensis transgenic embryogenic callus lines by Agrobacterium tumefaciens: roles of calcium.
Article in Plant cell reports, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 63 citations in OpenAlex.
- Structural and Functional Annotation of Transposable Elements Revealed a Potential Regulation of Genes Involved in Rubber Biosynthesis by TE-Derived siRNA Interference inInternational journal of molecular sciences · 2020Article
- Somatic Embryogenesis as Key Technology for Shaping the Rubber Tree of the Future.Frontiers in plant science · 2018Review
- Efficient regeneration and improved sonication-assisted Agrobacterium transformation (SAAT) method for Catharanthus roseus.3 Biotech · 2017Article
- Gain-of-function inSpringerPlus · 2016Article
- Overexpression of aFrontiers in plant science · 2016Article
- The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.Physiology and molecular biology of plants : an international journal of functional plant biology · 2014Article
- Over-expression of a cytosolic isoform of the HbCuZnSOD gene in Hevea brasiliensis changes its response to a water deficit.Plant molecular biology · 2012Article
- RNA-Seq analysis and de novo transcriptome assembly of Hevea brasiliensis.Plant molecular biology · 2011Article
- Variation in GUS activity in vegetatively propagated Hevea brasiliensis transgenic plants.Plant cell reports · 2011Article
- The green fluorescent protein as an efficient selection marker for Agrobacterium tumefaciens-mediated transformation in Hevea brasiliensis (Müll. Arg).Plant cell reports · 2010Article
- Pre- and post-agroinfection strategies for efficient leaf disk transformation and regeneration of transgenic strawberry plants.Plant cell reports · 2010Article
- A high-throughput Agrobacterium-mediated transformation system for the grass model species Brachypodium distachyon L.Transgenic research · 2008Article
- Efficient Agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Müll Arg. plants.Plant cell reports · 2006Article
- Agrobacterium-mediated transformation of the wetland monocot Typha latifolia L. (Broadleaf cattail).Plant cell reports · 2005Article
- A rapid and highly efficient method for transformation of sugarcane callus.Molecular biotechnology · 2004Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A procedure has been established for Agrobacterium tumefaciens-mediated genetic transformation of Hevea brasiliensis embryogenic friable calli. Precultivation of tissues on a CaCl(2)-free maintenance medium dramatically enhanced the transient activity of the reporter gene, gusA encoding beta-glucuronidase (GUS). The increase was first noticed in highly active cells (undifferentiated or/and embryogenic), in tissues precultured for 2-8 weeks. Beyond 8 weeks of preculture, GUS activity increased again, but this time in tissues consisting of differentiated cells accumulating polyphenols. Out of five Agrobacterium strains cocultivated with CaCl(2)-free precultured tissues, only inoculation with EHA105pC2301 led to high transient GUS activity. Paromomycin proved more effective than kanamycin for the selection of transformed cells, as it inhibits the growth of non-transformed cells more radically. Five paromomycin-resistant callus lines were established. The presence of gusA and neomycin phosphotransferase ( nptII) genes in the plant genome was confirmed by DNA amplification, and by Southern hybridization. These results confirmed that A. tumefaciens is an effective system for mediating stable transformation of rubber tree calli with a low copy number of transgenes. Transgenic callus lines constitute a useful tool for studying genes of interest on a cellular level and for regenerating transgenic rubber trees.
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