ArticlePlant cell reports2003
Selectable marker-free transgenic barley producing a high level of cellulase (1,4-beta-glucanase) in developing grains.
Article in Plant cell reports, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Genetic engineering of grass cell wall polysaccharides for biorefining.Plant biotechnology journal · 2017Review
- Transgenic Plant-Produced Hydrolytic Enzymes and the Potential of Insect Gut-Derived Hydrolases for Biofuels.Frontiers in plant science · 2016Review
- TaHsfA6f is a transcriptional activator that regulates a suite of heat stress protection genes in wheat (Triticum aestivum L.) including previously unknown Hsf targets.Journal of experimental botany · 2015Article
- The heat shock factor family from Triticum aestivum in response to heat and other major abiotic stresses and their role in regulation of heat shock protein genes.Journal of experimental botany · 2014Article
- Improved recombinant cellulase expression in chloroplast of tobacco through promoter engineering and 5' amplification promoting sequence.Plant molecular biology · 2013Article
- Article
- TaMYB13-1, a R2R3 MYB transcription factor, regulates the fructan synthetic pathway and contributes to enhanced fructan accumulation in bread wheat.Journal of experimental botany · 2013Article
- Hyperthermophilic endoglucanase for in planta lignocellulose conversion.Biotechnology for biofuels · 2012Article
- From miracle fruit to transgenic tomato: mass production of the taste-modifying protein miraculin in transgenic plants.Plant cell reports · 2012Review
- Plant carbohydrate binding module enhances activity of hybrid microbial cellulase enzyme.Frontiers in plant science · 2012Article
- High-level accumulation of recombinant miraculin protein in transgenic tomatoes expressing a synthetic miraculin gene with optimized codon usage terminated by the native miraculin terminator.Plant cell reports · 2011Article
- Barley genomics: An overview.International journal of plant genomics · 2008Article
- A novel cis-acting element, ESP, contributes to high-level endosperm-specific expression in an oat globulin promoter.Plant molecular biology · 2006Article
- Green fluorescent protein as a vital elimination marker to easily screen marker-free transgenic progeny derived from plants co-transformed with a double T-DNA binary vector system.Plant cell reports · 2005Article
- A synthetic xylanase as a novel reporter in plants.Plant cell reports · 2003Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of barley grains as bioreactors for high-level production of cellulase (1,4-beta-glucanase) was investigated. A hybrid cellulase gene, cel-hyb1, driven by the rice GluB-1 promoter was expressed specifically in developing endosperm. Codon usage optimisation of cel-hyb1 increased its expression in barley grains 527-fold and led to cellulase production of up to 1.5% of total grain protein. CEL-HYB1 enzyme in barley grains was highly stable during post-harvest storage. Selectable marker gene ( hph) was subsequently eliminated from transgenic lines through segregation of hph from synthetic cel-hyb1 ( syn.cel-hyb1) in T1 progeny, using a binary plasmid containing hph and syn.cel-hyb1 in separate T-DNAs. These data suggest that barley grains can potentially be used for the commercial production of cellulase.
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