ArticlePlant cell reports2003
Agrobacterium-mediated large-scale transformation of wheat (Triticum aestivum L.) using glyphosate selection.
Article in Plant cell reports, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed.
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- Latin America: a hub for agrobiotechnological innovations.Annals of botany · 2025Review
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- Highly EfficientPlants (Basel, Switzerland) · 2024Article
- An integrated strategy involving high-throughput sequencing to characterize an unknown GM wheat event in Canada.Plant biotechnology journal · 2024Article
- Establishment of an Efficient Sugarcane Transformation System via Herbicide-Resistant CP4-EPSPS Gene Selection.Plants (Basel, Switzerland) · 2024Article
- A highly efficient genetic transformation system for broccoli and subcellular localization.Frontiers in plant science · 2023Article
- Article
- An Outlook on Global Regulatory Landscape for Genome-Edited Crops.International journal of molecular sciences · 2021Review
- Agrobacterium-Mediated Genetic Transformation of the Medicinal PlantPlants (Basel, Switzerland) · 2020Article
- A simple and fastPlant methods · 2020Article
- gRNA validation for wheat genome editing with the CRISPR-Cas9 system.BMC biotechnology · 2019Article
- A quick, easy and cost-effective3 Biotech · 2019Article
- Optimization of Agrobacterium-mediated transformation in spring bread wheat using mature and immature embryos.Molecular biology reports · 2019Article
- Expression of the high molecular weight glutenin 1Ay gene from Triticum urartu in barley.Transgenic research · 2019Article
- An efficient and reproduciblePlant methods · 2019Article
- Particle bombardment-mediated co-transformation of theData in brief · 2018Article
- Cloning and characterization of TaVIP2 gene from Triticum aestivum and functional analysis in Nicotiana tabacum.Scientific reports · 2016Article
- Screening Auxin Response, In Vitro Culture Aptitude and Susceptibility to Agrobacterium-Mediated Transformation of Italian Commercial Durum Wheat Varieties.Molecules (Basel, Switzerland) · 2016Article
- Acetosyringone, pH and temperature effects on transient genetic transformation of immature embryos of Brazilian wheat genotypes by Agrobacterium tumefaciens.Genetics and molecular biology · 2015Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An Agrobacterium-mediated transformation system with glyphosate selection has been developed for the large-scale production of transgenic plants. The system uses 4-day precultured immature embryos as explants. A total of 30 vectors containing the 5-enol-pyruvylshikimate-3-phosphate synthase gene from Agrobacterium strain CP4 (aroA:CP4), which confers resistance to glyphosate, were introduced into wheat using this system. The aroA:CP4 gene served two roles in this study-selectable marker and gene of interest. More than 3,000 transgenic events were produced with an average transformation efficiency of 4.4%. The entire process from isolation of immature embryos to production of transgenic plantlets was 50-80 days. Transgenic events were evaluated over several generations based on genetic, agronomic and molecular criteria. Forty-six percent of the transgenic events fit a 3:1 segregation ratio. Molecular analysis confirmed that four of six lead transgenic events selected from Agrobacterium transformation contained a single insert and a single copy of the transgene. Stable expression of theAROA:CP4 gene was confirmed by ELISA through nine generations. A comparison of Agrobacterium-mediated transformation to a particle bombardment system demonstrated that the Agrobacterium system is reproducible, has a higher transformation efficiency with glyphosate selection and produces higher quality transgenic events in wheat. One of the lead events from this study, no. 33391, has been identified as a Roundup Ready wheat commercial candidate.
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