ArticleThe Journal of general physiology2003
Ca2+ sparks and embers of mammalian muscle. Properties of the sources.
Article in The Journal of general physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Novel Ca2+ wave mechanisms in cardiac myocytes revealed by multiscale Ca2+ release model.The Journal of general physiology · 2025Article
- Elementary calcium release events in the skeletal muscle cells of the honey bee Apis mellifera.Scientific reports · 2021Article
- Integrating Bioelectrical Currents and CaBioelectricity · 2020Review
- High Time Resolution Analysis of Voltage-Dependent and Voltage-Independent Calcium Sparks in Frog Skeletal Muscle Fibers.Frontiers in physiology · 2020Article
- Extraocular muscle function is impaired inThe Journal of general physiology · 2019Article
- Localized nuclear and perinuclear Ca(2+) signals in intact mouse skeletal muscle fibers.Frontiers in physiology · 2015Article
- Assessment of calcium sparks in intact skeletal muscle fibers.Journal of visualized experiments : JoVE · 2014Article
- Analysis of osmotic stress induced Ca2+ spark termination in mammalian skeletal muscle.Indian journal of biochemistry & biophysics · 2013Article
- Isoproterenol increases the fraction of spark-dependent RyR-mediated leak in ventricular myocytes.Biophysical journal · 2013Article
- Coupled gating of skeletal muscle ryanodine receptors is modulated by Ca2+, Mg2+, and ATP.American journal of physiology. Cell physiology · 2012Article
- Synthetic localized calcium transients directly probe signalling mechanisms in skeletal muscle.The Journal of physiology · 2012Article
- Properties of Ca2+ sparks revealed by four-dimensional confocal imaging of cardiac muscle.The Journal of general physiology · 2012Article
- Detection of calcium sparks in intact and permeabilized skeletal muscle fibers.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Reciprocal dihydropyridine and ryanodine receptor interactions in skeletal muscle activation.Journal of muscle research and cell motility · 2011Review
- Ryanodol action on calcium sparks in ventricular myocytes.Pflugers Archiv : European journal of physiology · 2010Article
- Ca sparks do not explain all ryanodine receptor-mediated SR Ca leak in mouse ventricular myocytes.Biophysical journal · 2010Article
- New factors contributing to dynamic calcium regulation in the skeletal muscle triad-a crowded place.Biophysical reviews · 2010Review
- Hyperactive intracellular calcium signaling associated with localized mitochondrial defects in skeletal muscle of an animal model of amyotrophic lateral sclerosis.The Journal of biological chemistry · 2010Article
- Indo-1 derivatives for local calcium sensing.ACS chemical biology · 2009Article
- Local calcium signals induced by hyper-osmotic stress in mammalian skeletal muscle cells.Journal of muscle research and cell motility · 2009Article
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6 authors.
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Abstract
Ca2+ sparks of membrane-permeabilized rat muscle cells were analyzed to derive properties of their sources. Most events identified in longitudinal confocal line scans looked like sparks, but 23% (1,000 out of 4,300) were followed by long-lasting embers. Some were preceded by embers, and 48 were "lone embers." Average spatial width was approximately 2 microm in the rat and 1.5 microm in frog events in analogous solutions. Amplitudes were 33% smaller and rise times 50% greater in the rat. Differences were highly significant. The greater spatial width was not a consequence of greater open time of the rat source, and was greatest at the shortest rise times, suggesting a wider Ca2+ source. In the rat, but not the frog, spark width was greater in scans transversal to the fiber axis. These features suggested that rat spark sources were elongated transversally. Ca2+ release was calculated in averages of sparks with long embers. Release current during the averaged ember started at 3 or 7 pA (depending on assumptions), whereas in lone embers it was 0.7 or 1.3 pA, which suggests that embers that trail sparks start with five open channels. Analysis of a spark with leading ember yielded a current ratio ranging from 37 to 160 in spark and ember, as if 37-160 channels opened in the spark. In simulations, 25-60 pA of Ca2+ current exiting a point source was required to reproduce frog sparks. 130 pA, exiting a cylindric source of 3 microm, qualitatively reproduced rat sparks. In conclusion, sparks of rat muscle require a greater current than frog sparks, exiting a source elongated transversally to the fiber axis, constituted by 35-260 channels. Not infrequently, a few of those remain open and produce the trailing ember.
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