ArticleJournal of clinical pathology2003
Study of viral integration of HPV-16 in young patients with LSIL.
Article in Journal of clinical pathology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 63 citations in OpenAlex.
- Exploring the association between the lineages of human papillomavirus type 16 and viral physical status in the development of cervical cancer in Iran.Scientific reports · 2025Article
- The role of HR-HPV integration in the progression of premalignant lesions into different cancer types.Heliyon · 2024Review
- An increase of microRNA-16-1 is associated with the high proliferation of squamous intraepithelial lesions in the presence of the integrated state of HR-HPV in liquid cytology samples.Oncology letters · 2020Article
- Progression Low Squamous Intraepithelial Lesion and Human Papillomavirus Infections.Materia socio-medica · 2020Article
- Methylation of the L1 gene and integration of human papillomavirus 16 and 18 in cervical carcinoma and premalignant lesions.Oncology letters · 2018Article
- Transcriptionally active regions are the preferred targets for chromosomal HPV integration in cervical carcinogenesis.PloS one · 2015Article
- Chromogenic in situ hybridization and p16/Ki67 dual staining on formalin-fixed paraffin-embedded cervical specimens: correlation with HPV-DNA test, E6/E7 mRNA test, and potential clinical applications.BioMed research international · 2013Article
- The integration of HR-HPV increases the expression of cyclins A and E in cytologies with and without low-grade lesions.Journal of cytology · 2013Article
- A review of methods for detect human Papillomavirus infection.Virology journal · 2012Review
- Genomic amplification patterns of human telomerase RNA gene and C-MYC in liquid-based cytological specimens used for the detection of high-grade cervical intraepithelial neoplasia.Diagnostic pathology · 2012Article
- Detection of HPV by in situ hybridization in thin-layer (ThinPrep) cervicovaginal samples.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2011Article
- Increase in viral load, viral integration, and gain of telomerase genes during uterine cervical carcinogenesis can be simultaneously assessed by the HPV 16/18 MLPA-assay.The American journal of pathology · 2010Article
- The physical state of HPV16 infection and its clinical significance in cancer precursor lesion and cervical carcinoma.Journal of cancer research and clinical oncology · 2008Article
- Analysis of human papillomavirus type 16 (HPV16) DNA load and physical state for identification of HPV16-infected women with high-grade lesions or cervical carcinoma.Journal of clinical microbiology · 2008Article
- Early integration of high copy HPV16 detectable in women with normal and low grade cervical cytology and histology.Journal of clinical pathology · 2006Article
- Human papillomavirus type 16 integration in cervical carcinoma in situ and in invasive cervical cancer.Journal of clinical microbiology · 2006Article
- p16INK4a expression and progression risk of low-grade intraepithelial neoplasia of the cervix uteri.Virchows Archiv : an international journal of pathology · 2004Article
- Human papillomavirus oncogenic expression in the dysplastic portio; an investigation of biopsies from 190 cervical cones.British journal of cancer · 2004Article
- Laboratory and clinical aspects of human papillomavirus testing.Critical reviews in clinical laboratory sciencesReview
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo investigate the physical status of human papillomavirus 16 (HPV-16) in low grade squamous intraepithelial lesions (LSILs) as a means of determining the percentage of viral integration.
methodsNinety two LSIL/HPV positive Thin Prep(TM) samples were initially tested for the E6 gene by the polymerase chain reaction (PCR) to identify the HPV-16 virus. To avoid false positive results, the specificity of the bands obtained from PCR was confirmed by Southern blot hybridisation with internal oligonucleotide probes. Next, a PCR screen for the E2 gene was performed to identify those samples in which the virus was integrated. Viral integration was detected in just over half of them.
resultsTwenty of the 92 samples were HPV-16 positive, as shown by PCR for the E6 gene. Southern blot analysis confirmed that 13 of these samples were positive for the viral E6 gene. Thus, viral integration was detected in just over a half of the samples positive for HPV-16.
conclusionsThese data show that HPV-16 integration occurs in a subset of LSILs. The measurement of HPV-16 integration would be a helpful complementary tool for cytological evaluation in primary cervical screening to identify those patients at risk of developing high grade squamous intraepithelial lesions and cervical cancer.
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