ArticleProceedings of the National Academy of Sciences of the United States of America2003
Functional reconstitution of COPI coat assembly and disassembly using chemically defined components.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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Who cites it
37 citing papers in PubMed, 81 citations in OpenAlex.
- ARF GTPases Function at the Golgi.Sub-cellular biochemistry · 2026Review
- Elucidating a novel metabolic pathway for enhanced antimicrobial glycolipid biosurfactant production in the yeast Meyerozyma guilliermondii.Scientific reports · 2025Article
- STED imaging of endogenously tagged ARF GTPases reveals their distinct nanoscale localizations.The Journal of cell biology · 2023Article
- Kinetics of Arf1 inactivation regulates Golgi organisation and function in non-adherent fibroblasts.Biology open · 2023Article
- An interaction between β'-COP and the ArfGAP, Glo3, maintains post-Golgi cargo recycling.The Journal of cell biology · 2023Article
- Dominant ARF3 variants disrupt Golgi integrity and cause a neurodevelopmental disorder recapitulated in zebrafish.Nature communications · 2022Article
- The Golgi complex: a hub of the secretory pathway.BMB reports · 2021Review
- The Glo3 GAP crystal structure supports the molecular niche model for ArfGAPs in COPI coats.Advances in biological regulation · 2021Article
- ARF GTPases and Their Ubiquitous Role in Intracellular Trafficking Beyond the Golgi.Frontiers in cell and developmental biology · 2021Review
- Review
- Golgi phosphoprotein 3 triggers signal-mediated incorporation of glycosyltransferases into coatomer-coated (COPI) vesicles.The Journal of biological chemistry · 2014Article
- Dissecting functions of the conserved oligomeric Golgi tethering complex using a cell-free assay.Traffic (Copenhagen, Denmark) · 2014Article
- COPI budding within the Golgi stack.Cold Spring Harbor perspectives in biology · 2011Review
- Several ADP-ribosylation factor (Arf) isoforms support COPI vesicle formation.The Journal of biological chemistry · 2011Article
- Focusing on Arf GAPs.Cellular logistics · 2011Article
- GAPs: Terminator versus effector functions and the role(s) of ArfGAP1 in vesicle biogenesis.Cellular logistics · 2011Article
- Role of ArfGAP1 in COPI vesicle biogenesis.Cellular logistics · 2011Article
- GAPs in the context of COPI: Enzymes, coat components or both?Cellular logistics · 2011Article
- Models for the functions of Arf GAPs.Seminars in cell & developmental biology · 2011Review
- ARFGAP2 and ARFGAP3 are essential for COPI coat assembly on the Golgi membrane of living cells.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coat protein I (COPI)-coated transport vesicles mediate protein and lipid transport in the early secretory pathway. The basic machinery required for the formation of these transport intermediates has been elucidated based on the reconstitution of COPI-coated vesicle formation from chemically defined liposomes. In this experimental system, the coat components coatomer and GTP-bound ADP-ribosylation factor (ARF), as well as p23 as a membrane-bound receptor for COPI coat proteins, were shown to be both necessary and sufficient to promote COPI-coated vesicle formation. Based on biochemical and ultrastructural analyses, we now demonstrate that the catalytic domain of ARF-GTPase-activating protein (GAP) alone is sufficient to initiate uncoating of liposome-derived COPI-coated vesicles. By contrast, ARF-GAP activity is not required for COPI coat assembly and, therefore, does not seem to represent an essential coat component of COPI vesicles as suggested recently [Yang, J. S., Lee, S. Y., Gao, M., Bourgoin, S., Randazzo, P. A., et al. (2002) J. Cell Biol. 159, 69-78]. Thus, a complete round of COPI coat assembly and disassembly has been reconstituted with purified components defining the core machinery of COPI vesicle biogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.