ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2003
Bone marrow-derived cells expressing green fluorescent protein under the control of the glial fibrillary acidic protein promoter do not differentiate into astrocytes in vitro and in vivo.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed.
- Human dental stem cell derived transgene-free iPSCs generate functional neurons via embryoid body-mediated and direct induction methods.Journal of tissue engineering and regenerative medicine · 2018Article
- Finding degrees of separation: experimental approaches for astroglial and oligodendroglial cell isolation and genetic targeting.Journal of neuroscience methods · 2014Review
- VEGF induces neuroglial differentiation in bone marrow-derived stem cells and promotes microglia conversion following mobilization with GM-CSF.Stem cell reviews and reports · 2012Article
- CD36 in the periphery and brain synergizes in stroke injury in hyperlipidemia.Annals of neurology · 2012Article
- Cellular changes underlying hyperoxia-induced delay of white matter development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Brain APCs including microglia are only differential and positional polymorphs.Annals of neurosciences · 2010Review
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- Robust in vivo transduction of nervous system and neural stem cells by early gestational intra amniotic gene transfer using lentiviral vector.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Stem cells for ischemic brain injury: a critical review.The Journal of comparative neurology · 2009Review
- Profiling of astrocyte properties in the hyperammonaemic brain: shedding new light on the pathophysiology of the brain damage in hyperammonaemia.Journal of inherited metabolic disease · 2008Review
- Article
- Multipotent skin-derived precursors: adult neural crest-related precursors with therapeutic potential.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2008Review
- A rose by any other name? The potential consequences of microglial heterogeneity during CNS health and disease.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2007Review
- Article
- Toll-like receptor 4 on nonhematopoietic cells sustains CNS inflammation during endotoxemia, independent of systemic cytokines.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Robert Feulgen Prize Lecture. Grenzgänger: adult bone marrow cells populate the brain.Histochemistry and cell biology · 2003Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Differentiation of bone marrow (BM) cells into astroglia expressing the glial fibrillary acidic protein (GFAP) has been reported in vitro and after intracerebral or systemic BM transplantation. In contrast, recent data suggest that astrocytic differentiation does not occur from BM-derived cells in vivo. Using transgenic mice that express the enhanced green fluorescent protein (GFP) under the control of the human glial fibrillary acidic protein (GFAP) promoter, we investigated the potential of adult murine BM-derived cells to differentiate into macroglia. In the brains of GFAP-GFP transgenic mice, astrocytes were brightly fluorescent from the expression of GFP. When BM from these animals was transplanted into lethally irradiated wild-type animals, the transgene was detected in the reconstituted hematopoietic system, but no GFP expression was found in the nervous system. In contrast, GFAP-GFP neuroectodermal anlage grafted into adult wild-type striatum gave rise to GFP-expressing astrocytes. Because cerebral ischemia has been suggested to promote the differentiation of BM-derived cells into astrocytes, BM chimeric mice were subjected to focal cerebral ischemia. No GFP-positive cells were found in the ischemic or contralateral hemispheres of these brains. Even after direct injection of GFAP-GFP transgenic BM cells into wild-type striatum, no GFP-expressing astroglia were detected. To test the hypothesis that the in vitro environment might be more permissible for astroglial differentiation, we cultured BM from mice that constitutively express GFP, BM cells expressing GFP from a retroviral vector, and BM from GFAP-GFP transgenic mice on astrocytes and on organotypic hippocampal slices. In all experimental paradigms, BM-derived cells were found to differentiate into ramified microglia but not into GFAP-expressing astrocytes.
Indexed as
Identifiers
12832523PMC6741150What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.