ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2003
A novel antigen sensitive to calcium chelation that is associated with the tip links and kinocilial links of sensory hair bundles.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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Who cites it
37 citing papers in PubMed, 83 citations in OpenAlex.
- Mechanotransduction in mammalian sensory hair cells.Molecular and cellular neurosciences · 2022Review
- Hair-Bundle Links: Genetics as the Gateway to Function.Cold Spring Harbor perspectives in medicine · 2019Review
- Beyond Cell-Cell Adhesion: Sensational Cadherins for Hearing and Balance.Cold Spring Harbor perspectives in biology · 2018Review
- FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Tuning Inner-Ear Tip-Link Affinity Through Alternatively Spliced Variants of Protocadherin-15.Biochemistry · 2018Article
- Functional Analysis of the Transmembrane and Cytoplasmic Domains of Pcdh15a in Zebrafish Hair Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Activity-Dependent Phosphorylation by CaMKIIδ Alters the CaFrontiers in synaptic neuroscience · 2017Article
- Article
- In vivo physiological recording from the lateral line of juvenile zebrafish.The Journal of physiology · 2016Article
- Transduction without tip links in cochlear hair cells is mediated by ion channels with permeation properties distinct from those of the mechano-electrical transducer channel.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Molecular architecture of the chick vestibular hair bundle.Nature neuroscience · 2013Article
- Molecular remodeling of tip links underlies mechanosensory regeneration in auditory hair cells.PLoS biology · 2013Article
- Biomechanical measurement of kinocilium.Methods in enzymology · 2013Article
- Localization of Usher 1 proteins to the photoreceptor calyceal processes, which are absent from mice.The Journal of cell biology · 2012Article
- Kinocilia mediate mechanosensitivity in developing zebrafish hair cells.Developmental cell · 2012Article
- Current understanding of usher syndrome type II.Frontiers in bioscience (Landmark edition) · 2012Article
- Mutations in protocadherin 15 and cadherin 23 affect tip links and mechanotransduction in mammalian sensory hair cells.PloS one · 2011Article
- Biomechanics of hair cell kinocilia: experimental measurement of kinocilium shaft stiffness and base rotational stiffness with Euler-Bernoulli and Timoshenko beam analysis.The Journal of experimental biology · 2011Article
- Identification of the hair cell soma-1 antigen, HCS-1, as otoferlin.Journal of the Association for Research in Otolaryngology : JARO · 2010Article
- Asymmetric distribution of cadherin 23 and protocadherin 15 in the kinocilial links of avian sensory hair cells.The Journal of comparative neurology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Tip links are extracellular, cell-surface-associated filaments of unknown molecular composition that are thought to gate the mechanotransducer channel of the sensory hair cell. They disappear from the hair bundle in response to calcium chelation and lanthanum treatment and resist degradation by the protease subtilisin. A monoclonal antibody derived from a hybridoma screen identified a novel antigen associated with tip links, the tip-link antigen. The tip-link antigen is also associated with kinocilial links, subtilisin-resistant filaments that are sensitive to calcium chelation and connect the kinocilium to the tallest stereocilia of the hair bundle. Furthermore, the tip-link antigen is expressed in the retina, where it is associated with the ciliary calyx, a ring of microvilli that surrounds the outer segment of the photoreceptor. The tip-link antigen rapidly disappears from the surface of the hair bundle in response to calcium chelation. It is also subtilisin resistant, relative to the ankle-link antigen, an antigen associated with another type of hair bundle link. The tip-link antigen is lanthanum sensitive and, like tip links, reappears on the surface of the hair bundle after calcium chelation. The monoclonal antibody to the tip-link antigen immunoprecipitates two concanavalin A-reactive polypeptides with apparent molecular masses of 200 and 250 kDa from detergent extracts of the retina. These results provide the first identification of a cell surface antigen associated with tip links, indicate that tip links share properties in common with kinocilial links, and reveal a second epitope that, along with the ankle-link antigen, is common to both sensory hair bundles and the ciliary calyx of photoreceptors.
Indexed as
Identifiers
12832510PMC6741187W2122929023What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.