ArticleThe Journal of physiology2003
Rat pancreas secretes particulate ecto-nucleotidase CD39.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Purinergic Signaling in Pancreas-From Physiology to Therapeutic Strategies in Pancreatic Cancer.International journal of molecular sciences · 2020Review
- The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells.Pflugers Archiv : European journal of physiology · 2016Article
- ATP release, generation and hydrolysis in exocrine pancreatic duct cells.Purinergic signalling · 2015Article
- Rat submandibular glands secrete nanovesicles with NTPDase and 5'-nucleotidase activities.Purinergic signalling · 2015Article
- Role of vesicular nucleotide transporter VNUT (SLC17A9) in release of ATP from AR42J cells and mouse pancreatic acinar cells.Purinergic signalling · 2014Article
- Purinergic receptors and nucleotide processing ectoenzymes: Their roles in regulating mesenchymal stem cell functions.World journal of stem cells · 2014Review
- Acid-base transport in pancreas-new challenges.Frontiers in physiology · 2013Review
- Cellular function and molecular structure of ecto-nucleotidases.Purinergic signalling · 2012Review
- Metabolism of circulating ADP in the bloodstream is mediated via integrated actions of soluble adenylate kinase-1 and NTPDase1/CD39 activities.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Pancreatic bicarbonate secretion involves two proton pumps.The Journal of biological chemistry · 2011Article
- Effect of P2X(7) receptor knockout on exocrine secretion of pancreas, salivary glands and lacrimal glands.The Journal of physiology · 2010Article
- Localization of plasma membrane bound NTPDases in the murine reproductive tract.Histochemistry and cell biology · 2009Article
- Purinergic receptors in the endocrine and exocrine pancreas.Purinergic signalling · 2008Article
- Intravascular ADP and soluble nucleotidases contribute to acute prothrombotic state during vigorous exercise in humans.The Journal of physiology · 2007Article
- The E-NTPDase family of ectonucleotidases: Structure function relationships and pathophysiological significance.Purinergic signalling · 2006Article
- Invited Lectures : Overviews Purinergic signalling: past, present and future.Purinergic signalling · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In exocrine pancreas, acini release ATP and the excurrent ducts express several types of purinergic P2 receptors. Thereby, ATP, or its hydrolytic products, might play a role as a paracrine regulator between acini and ducts. The aim of the present study was to elucidate whether this acinar-ductal signalling is regulated by nucleotidase(s), and to characterize and localize one of the nucleotidases within the rat pancreas. Using RT-PCR and Western blotting we show that pancreas expresses the full length ecto-nucleoside triphosphate diphosphohydrolase, CD39. Immunofluorescence shows CD39 localization on basolateral membranes of acini and intracellularly. In small intercalated/ interlobular ducts, CD39 immunofluorescence was localized on the luminal membranes, while in larger ducts it was localized on the basolateral membranes. Upon stimulation with cholecystokinin-octapeptide-8 (CCK-8), acinar CD39 relocalizes in clusters towards the lumen and is secreted. As a result, pancreatic juice collected from intact pancreas stimulated with CCK-8 contained nucleotidase activity, including that of CD39, and no detectable amounts of ATP. Anti-CD39 antibodies detected the full length (78 kDa) CD39 in pancreatic juice. This CD39 was confined only to the particulate and not to the soluble fraction of CCK-8-stimulated secretion. No CD39 activity was detected in secretion stimulated by secretin. The role of secreted particulate, possibly microsomal, CD39 would be to regulate intraluminal ATP concentrations within the ductal tree. In conclusion, we show a novel inducible release of full length particulate CD39, and propose its role in the physiological context of pancreatic secretion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.