ArticleJournal of virology2003
The tailless icosahedral membrane virus PRD1 localizes the proteins involved in genome packaging and injection at a unique vertex.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed.
- Integrative Approaches to Study Virus Structures.Sub-cellular biochemistry · 2024Review
- Chronic Release of Tailless Phage Particles from Lactococcus lactis.Applied and environmental microbiology · 2022Article
- Half a Century of Research on Membrane-Containing Bacteriophages: Bringing New Concepts to Modern Virology.Viruses · 2019Review
- Review
- Capsid-deficient alphaviruses generate propagative infectious microvesicles at the plasma membrane.Cellular and molecular life sciences : CMLS · 2016Article
- A structural model of the genome packaging process in a membrane-containing double stranded DNA virus.PLoS biology · 2014Article
- Article
- Structure and assembly of complex viruses.Sub-cellular biochemistry · 2013Review
- Identification and characterization of a DNA binding domain on the adenovirus IVa2 protein.Virology · 2012Article
- Condensed genome structure.Advances in experimental medicine and biology · 2012Review
- Structures of giant icosahedral eukaryotic dsDNA viruses.Current opinion in virology · 2011Review
- Bacteriophage assembly.Viruses · 2011Review
- In vivo assembly of an archaeal virus studied with whole-cell electron cryotomography.Structure (London, England : 1993) · 2010Article
- Particle assembly and ultrastructural features associated with replication of the lytic archaeal virus sulfolobus turreted icosahedral virus.Journal of virology · 2009Article
- Structure of bacteriophage SPP1 head-to-tail connection reveals mechanism for viral DNA gating.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Structural studies of the giant mimivirus.PLoS biology · 2009Article
- Presence of the adenovirus IVa2 protein at a single vertex of the mature virion.Journal of virology · 2008Article
- Distinct DNA exit and packaging portals in the virus Acanthamoeba polyphaga mimivirus.PLoS biology · 2008Article
- Genome sequence, structural proteins, and capsid organization of the cyanophage Syn5: a "horned" bacteriophage of marine synechococcus.Journal of molecular biology · 2007Article
- Tale of two spikes in bacteriophage PRD1.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The double-stranded DNA (dsDNA) virus PRD1 carries its genome in a membrane surrounded by an icosahedral protein shell. The shell contains 240 copies of the trimeric P3 protein arranged with a pseudo T = 25 triangulation that is reminiscent of the mammalian adenovirus. DNA packaging and infection are believed to occur through the vertices of the particle. We have used immunolabeling to define the distribution of proteins on the virion surface. Antibodies to protein P3 labeled the entire surface of the virus. Most of the 12 vertices labeled with antibodies directed against proteins P5, P2, and P31. These proteins are known to function in virus binding to the cell surface. Proteins P6, P11, and P20 were found on a single vertex per virion. The P6 and P20 proteins are believed to function in DNA packaging. Protein P11 is a pilot protein that is involved in a complex that mediates the early stages of DNA entry to the host cell. Labeling with antibodies to P5 or P2 did not affect the labeling of P6, the unique vertex protein. Labeling with antibodies to the unique vertex protein P6 interfered with the labeling by antibodies to the unique vertex protein P20. We conclude that PRD1 utilizes 11 of its vertices for initial receptor binding. It utilizes a single, unique vertex for both DNA packing during assembly and DNA delivery during infection.
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