ArticleBiophysical journal2003
Ca(2+)- and voltage-dependent gating of Ca(2+)- and ATP-sensitive cationic channels in brain capillary endothelium.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- Transient Receptor Potential Channels in the Healthy and Diseased Blood-Brain Barrier.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2024Review
- Transient Receptor Potential Vanilloid in the Brain Gliovascular Unit: Prospective Targets in Therapy.Pharmaceutics · 2021Review
- PIPeLife · 2018Article
- Structure of a TRPM2 channel in complex with CaeLife · 2018Article
- Fluid and ion transfer across the blood-brain and blood-cerebrospinal fluid barriers; a comparative account of mechanisms and roles.Fluids and barriers of the CNS · 2016Review
- TREK-king the blood-brain-barrier.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2014Review
- Identification of direct and indirect effectors of the transient receptor potential melastatin 2 (TRPM2) cation channel.The Journal of biological chemistry · 2010Article
- Kv1 and Kir2 potassium channels are expressed in rat brain endothelial cells.Pflugers Archiv : European journal of physiology · 2008Article
- Non-selective cation channels, transient receptor potential channels and ischemic stroke.Biochimica et biophysica acta · 2007Review
- Ca2+-dependent regulation of a non-selective cation channel from Aplysia bag cell neurones.The Journal of physiology · 2006Article
- Statistical evaluation of ion-channel gating models based on distributions of log-likelihood ratios.Biophysical journal · 2006Article
- Decavanadate modulates gating of TRPM4 cation channels.The Journal of physiology · 2004Article
- Antagonistic regulation of native Ca2+- and ATP-sensitive cation channels in brain capillaries by nucleotides and decavanadate.The Journal of general physiology · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biophysical properties of the Ca(2+)-activated nonselective cation channel expressed in brain capillaries were studied in inside-out patches from primary cultures of rat brain microvascular endothelial cells. At -40 mV membrane potential, open probability (P(o)) was activated by cytosolic [Ca(2+)] > 1 micro M and was half-maximal at approximately 20 micro M. Increasing [Ca(2+)] stimulated opening rate with little effect on closing rate. At constant [Ca(2+)], P(o) was voltage-dependent, and effective gating charge corresponded to 0.6 +/- 0.1 unitary charges. Depolarization accelerated opening and slowed closing, thereby increasing apparent affinity for Ca(2+). Within approximately 1 min of excision, P(o) declined to a lower steady state with decreased sensitivity toward activating Ca(2+) when studied at a fixed voltage, and toward activating voltage when studied at a fixed [Ca(2+)]. Deactivated channels opened approximately 5-fold slower and closed approximately 10-fold faster. The sulfhydryl-reducing agent dithiotreitol (1 mM) completely reversed acceleration of closing rate but failed to recover opening rate. Single-channel gating was complex; distributions of open and closed dwell times contained at least four and five exponential components, respectively. The longest component of the closed-time distribution was markedly sensitive to both [Ca(2+)] and voltage. We conclude that the biophysical properties of gating of this channel are remarkably similar to those of large-conductance Ca(2+)-activated K(+) channels.
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