ArticleBiophysical journal2003
The pore helix is involved in stabilizing the open state of inwardly rectifying K+ channels.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- The unique structural characteristics of the Kir 7.1 inward rectifier potassium channel: a novel player in energy homeostasis control.American journal of physiology. Cell physiology · 2023Review
- Novel Insertion Mutation in KCNJ5 Channel Produces Constitutive Aldosterone Release From H295R Cells.Molecular endocrinology (Baltimore, Md.) · 2015Article
- Temperature sensitivity of two-pore (K2P) potassium channels.Current topics in membranes · 2014Review
- Targeted molecular dynamics (TMD) of the full-length KcsA potassium channel: on the role of the cytoplasmic domain in the opening process.Journal of molecular modeling · 2013Article
- Metabolic and thermal stimuli control K(2P)2.1 (TREK-1) through modular sensory and gating domains.The EMBO journal · 2012Article
- Pore determinants of KCNQ3 K+ current expression.Biophysical journal · 2012Article
- A multipoint hydrogen-bond network underlying KcsA C-type inactivation.Biophysical journal · 2011Article
- Structural basis for the coupling between activation and inactivation gates in K(+) channels.Nature · 2010Article
- Article
- Modular strategy for the semisynthesis of a K+ channel: investigating interactions of the pore helix.ACS chemical biology · 2009Article
- Elucidation of the gating of the GIRK channel using a spectroscopic approach.The Journal of physiology · 2009Review
- Article
- Mutations reveal voltage gating of CNGA1 channels in saturating cGMP.The Journal of general physiology · 2009Article
- Long-pore electrostatics in inward-rectifier potassium channels.The Journal of general physiology · 2008Article
- A yeast genetic screen reveals a critical role for the pore helix domain in TRP channel gating.Neuron · 2008Article
- An inactivation gate in the selectivity filter of KCNQ1 potassium channels.Biophysical journal · 2007Article
- Uncoupling proton activation of vanilloid receptor TRPV1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Base of pore loop is important for rectification, activation, permeation, and block of Kir3.1/Kir3.4.Biophysical journal · 2006Article
- Concerted gating mechanism underlying KATP channel inhibition by ATP.Biophysical journal · 2004Article
- Evolving potassium channels by means of yeast selection reveals structural elements important for selectivity.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Ion channels can be gated by various extrinsic cues, such as voltage, pH, and second messengers. However, most ion channels display extrinsic cue-independent transitions as well. These events represent spontaneous conformational changes of the channel protein. The molecular basis for spontaneous gating and its relation to the mechanism by which channels undergo activation gating by extrinsic cue stimulation is not well understood. Here we show that the proximal pore helix of inwardly rectifying (Kir) channels is partially responsible for determining spontaneous gating characteristics, affecting the open state of the channel by stabilizing intraburst openings as well as the bursting state itself without affecting K(+) ion-channel interactions. The effect of the pore helix on the open state of the channel is qualitatively similar to that of two well-characterized mutations at the second transmembrane domain (TM2), which stabilize the channel in its activated state. However, the effects of the pore helix and the TM2 mutations on gating were additive and independent of each other. Moreover, in sharp contrast to the two TM2 mutations, the pore helix mutation did not affect the functionality of the agonist-responsive gate. Our results suggest that in Kir channels, the bottom of the pore helix and agonist-induced conformational transitions at the TM2 ultimately stabilize via different pathways the open conformation of the same gate.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.