ArticleBiophysical journal2003
Kinetics from nonequilibrium single-molecule pulling experiments.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers.
What it found
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Who cites it
118 citing papers in PubMed.
- Single-Molecule Force Spectroscopy: Experiments, Analysis, and Simulations.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Observation of Mechanical and Kinetic Distinctions between Individual Isoleucine and Arginine Residues in a Peptide Dissociating from a Model Lipid Bilayer.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Modeling Targeted Mechanochemistry in Polymeric Solids.Chemical reviews · 2026Review
- Membrane Protein Folding and Biogenesis: Insights from Single-Molecule Force Spectroscopy.Chemical reviews · 2026Review
- Computational Characterization of the Role of LEM2/LaminA Interactions on the Stability of BAF-Dimer Using Molecular Simulations.Proteins · 2026Article
- Computational Study of Terahertz-Induced Phase Transitions at Molecular Interfaces.Research (Washington, D.C.) · 2026Article
- Deciphering the sequence-dependent unfolding pathways of an RNA pseudoknot with steered molecular dynamics.Journal of computer-aided molecular design · 2025Article
- Chemifriction and Superlubricity: Friends or Foes?The journal of physical chemistry letters · 2025Article
- Polymer time crystal: Mechanical activation of reversible bonds by low-amplitude high frequency excitations.Science advances · 2024Article
- Two operational modes of atomic force microscopy reveal similar mechanical properties for homologous regions of dystrophin and utrophin.bioRxiv : the preprint server for biology · 2024Article
- Exploring Product Release from Yeast Cytosine Deaminase with Metadynamics.The journal of physical chemistry. B · 2024Article
- Microscopic theory, analysis, and interpretation of conductance histograms in molecular junctions.Nature communications · 2023Article
- Combining DNA scaffolds and acoustic force spectroscopy to characterize individual protein bonds.Biophysical journal · 2023Article
- Measuring pico-Newton Forces with Lipid Anchors as Force Sensors in Molecular Dynamics Simulations.The journal of physical chemistry. B · 2023Article
- Inferring equilibrium transition rates from nonequilibrium protocols.Biophysical journal · 2023Article
- Force Dependent Barriers from Analytic Potentials within Elastic Environments.Chemphyschem : a European journal of chemical physics and physical chemistry · 2022Article
- Force Dependence of Proteins' Transition State Position and the Bell-Evans Model.Nanomaterials (Basel, Switzerland) · 2021Article
- Dependence of Work on the Pulling Speed in Mechanical Ligand Unbinding.The journal of physical chemistry. B · 2021Article
- Nanomechanical Phenotypes in Cardiac Myosin-Binding Protein C Mutants That Cause Hypertrophic Cardiomyopathy.ACS nano · 2021Article
- The ribosome modulates folding inside the ribosomal exit tunnel.Communications biology · 2021Article
58 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Mechanical forces exerted by laser tweezers or atomic force microscopes can be used to drive rare transitions in single molecules, such as unfolding of a protein or dissociation of a ligand. The phenomenological description of pulling experiments based on Bell's expression for the force-induced rupture rate is found to be inadequate when tested against computer simulations of a simple microscopic model of the dynamics. We introduce a new approach of comparable complexity to extract more accurate kinetic information about the molecular events from pulling experiments. Our procedure is based on the analysis of a simple stochastic model of pulling with a harmonic spring and encompasses the phenomenological approach, reducing to it in the appropriate limit. Our approach is tested against computer simulations of a multimodule titin model with anharmonic linkers and then an illustrative application is made to the forced unfolding of I27 subunits of the protein titin. Our procedure to extract kinetic information from pulling experiments is simple to implement and should prove useful in the analysis of experiments on a variety of systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.