ArticleIn vitro cellular & developmental biology : journal of the Tissue Culture Association
A transformed human epithelial cell line that retains tight junctions post crisis.
Article in In vitro cellular & developmental biology : journal of the Tissue Culture Association. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
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- Sustained-Release Powders Based on Polymer Particles for Pulmonary Delivery of Beclomethasone Dipropionate in the Treatment of Lung Inflammation.Pharmaceutics · 2023Article
- The contribution of reticular basement membrane proteins to basal airway epithelial attachment, spreading and barrier formation: implications for airway remodeling in asthma.Frontiers in medicine · 2023Article
- Expression/Activation of PAR-1 in Airway Epithelial Cells of COPD Patients: Ex Vivo/In Vitro Study.International journal of molecular sciences · 2021Article
- Loading of Beclomethasone in Liposomes and Hyalurosomes Improved with Mucin as Effective Approach to Counteract the Oxidative Stress Generated by Cigarette Smoke Extract.Nanomaterials (Basel, Switzerland) · 2021Article
- Butyrate and Propionate Restore the Cytokine and House Dust Mite Compromised Barrier Function of Human Bronchial Airway Epithelial Cells.International journal of molecular sciences · 2020Article
- Coxsackievirus Type B3 Is a Potent Oncolytic Virus againstMolecular therapy oncolytics · 2019Article
- Phagocytosis ofFrontiers in cellular and infection microbiology · 2019Article
- Polyaspartamide-Based Nanoparticles Loaded with Fluticasone Propionate and the In Vitro Evaluation towards Cigarette Smoke Effects.Nanomaterials (Basel, Switzerland) · 2017Article
- CRISPLD2 (LGL1) inhibits proinflammatory mediators in human fetal, adult, and COPD lung fibroblasts and epithelial cells.Physiological reports · 2016Article
- Functional and cytometric examination of different human lung epithelial cell types as drug transport barriers.Archives of pharmacal research · 2016Article
- Protocadherin-1 Localization and Cell-Adhesion Function in Airway Epithelial Cells in Asthma.PloS one · 2016Article
- MD2 expression is reduced in large airways of smokers and COPD smokers.Molecular and cellular biochemistry · 2015Article
- Transcriptomic Analyses of the Biological Effects of Airborne PM2.5 Exposure on Human Bronchial Epithelial Cells.PloS one · 2015Article
- Article
- Comparative cytoprotective effects of carbocysteine and fluticasone propionate in cigarette smoke extract-stimulated bronchial epithelial cells.Cell stress & chaperones · 2013Article
- Cigarette smoke increases BLT2 receptor functions in bronchial epithelial cells: in vitro and ex vivo evidence.Immunology · 2013Article
- Host cell autophagy modulates early stages of adenovirus infections in airway epithelial cells.Journal of virology · 2013Article
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Authors and funding
10 authors.
Funding
Abstract
The successful establishment of a postcrisis SV-40 T antigen transformed epithelial cell line, 1HAEo-, which retains tight junctions and vectorial ion transport, is described. Immunocytochemical analysis of 1HAEo- cells shows a defined pattern of cytokeratin staining and a characteristic pericellular localization of the adhesion molecule cellCAM 120/80, indicating the presence of junctional complexes. The presence of both tight junctions and desmosomes has been confirmed by electron microscopy. Cell monolayers have good transepithelial resistance measured in Ussing chambers. Cells increase chloride ion transport in response to addition of agents that raise either intracellular cAMP or calcium, measured either by 36Cl- efflux or whole-cell patch clamp. An increase in short-circuit current, in response to these agents, can be measured in Ussing chambers. The presence of a depolarization-induced outwardly rectifying 45 pS chloride channel has been demonstrated in single cell detached membrane patches. In addition, the cells have been found to express mucin mRNA. These cells therefore demonstrate that it is possible to select transformed cell clones with particular morphologic characteristics, i.e. the presence of tight junctions and cell polarity, which also retain useful epithelial cell-specific functions, including vectorial ion transport. They also provide a major resource for the study of the structure and function of human epithelia.
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