ArticleMolecular genetics and genomics : MGG2003
Concomitant reiterative BAC walking and fine genetic mapping enable physical map development for the broad-spectrum late blight resistance region, RB.
Article in Molecular genetics and genomics : MGG, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 25 citations in OpenAlex.
- Introgression of Two Broad-Spectrum Late Blight Resistance Genes,Frontiers in plant science · 2020Article
- Evolutionary analysis of RB/Rpi-blb1 locus in the Solanaceae family.Molecular genetics and genomics : MGG · 2015Article
- A DArT marker-based linkage map for wild potato Solanum bulbocastanum facilitates structural comparisons between Solanum A and B genomes.BMC genetics · 2014Article
- Molecular mapping of vernalization requirement and fertility restoration genes in carrot.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Major-effect QTLs for stem and foliage resistance to late blight in the wild potato relatives Solanum sparsipilum and S. spegazzinii are mapped to chromosome X.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Characterization of a deep-coverage carrot (Daucus carota L.) BAC library and initial analysis of BAC-end sequences.Molecular genetics and genomics : MGG · 2009Article
- Sobo, a recently amplified satellite repeat of potato, and its implications for the origin of tandemly repeated sequences.Genetics · 2005Article
- Bacterial artificial chromosome-derived molecular markers for early bolting in sugar beet.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Transfer of tuber soft rot and early blight resistances from Solanum brevidens into cultivated potato.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2004Article
- BIBAC and TAC clones containing potato genomic DNA fragments larger than 100 kb are not stable in Agrobacterium.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2003Article
- Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The wild potato species Solanum bulbocastanum is a source of genes for potent late blight resistance. We previously mapped resistance to a single region of the S. bulbocastanum chromosome 8 and named the region RB (for "Resistance from S. Bulbocastanum"). We now report physical mapping and contig construction for the RB region via a novel reiterative method of BAC walking and concomitant fine genetic mapping. BAC walking was initiated using RFLP markers previously shown to be associated with late blight resistance. Subcontig extension was accomplished using new probes developed from BAC ends. Significantly, BAC end and partial BAC sequences were also used to develop PCR-based markers to enhance map resolution in the RB region. As they were developed from BAC clones of known position relative to RB, our PCR-based markers are known a priori to be physically closer to the resistance region. These markers allowed the efficient screening of large numbers of segregating progeny at the cotyledon stage, and permitted us to assign the resistance phenotype to a region of approximately 55 kb. Our markers also directed BAC walking efforts away from regions distantly related to RB in favor of the 55-kb region. Because the S. bulbocastanum genotype used in BAC library construction is heterozygous for RB (RB/rb), codominant PCR-based markers, originally developed for fine-scale mapping, were also used to determine homolog origins for individual BAC clones. Ultimately, BAC contigs were constructed for the RB region from both resistant (RB) and susceptible (rb) homologs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.