ArticlePlant cell reports2003
Efficient transformation of Medicago truncatula cv. Jemalong using the hypervirulent Agrobacterium tumefaciens strain AGL1.
Article in Plant cell reports, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Genetic transformation of forage crops: comparative barriers, evidence, and emerging strategies.Plant cell reports · 2026Review
- Nodule organogenesis in Medicago truncatula requires local stage-specific auxin biosynthesis and transport.Plant physiology · 2025Article
- Article
- ImprovingGenes · 2023Article
- Recalcitrance to transformation, a hindrance for genome editing of legumes.Frontiers in genome editing · 2023Review
- Article
- Article
- SNARE Complexity in Arbuscular Mycorrhizal Symbiosis.Frontiers in plant science · 2020Article
- Article
- Comparison of efficiency and time to regeneration ofPlant methods · 2019Article
- Analysis of Large Seeds from Three Different Medicago truncatula Ecotypes Reveals a Potential Role of Hormonal Balance in Final Size Determination of Legume Grains.International journal of molecular sciences · 2016Article
- PUB1 Interacts with the Receptor Kinase DMI2 and Negatively Regulates Rhizobial and Arbuscular Mycorrhizal Symbioses through Its Ubiquitination Activity in Medicago truncatula.Plant physiology · 2016Article
- Response of Medicago truncatula seedlings to colonization by Salmonella enterica and Escherichia coli O157:H7.PloS one · 2014Article
- Automated analysis of calcium spiking profiles with CaSA software: two case studies from root-microbe symbioses.BMC plant biology · 2013Article
- Medicago truncatula ERN transcription factors: regulatory interplay with NSP1/NSP2 GRAS factors and expression dynamics throughout rhizobial infection.Plant physiology · 2012Article
- An in planta, Agrobacterium-mediated transient gene expression method for inducing gene silencing in rice (Oryza sativa L.) leaves.Rice (New York, N.Y.) · 2012Article
- Backbone-free transformation of barrel medic (Medicago truncatula) with a Medicago-derived transfer DNA.Plant cell reports · 2010Article
- A remorin protein interacts with symbiotic receptors and regulates bacterial infection.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Osmotic shock improves Tnt1 transposition frequency in Medicago truncatula cv Jemalong during in vitro regeneration.Plant cell reports · 2009Article
- Agrobacterium rhizogenes-mediated transformation of Superroot-derived Lotus corniculatus plants: a valuable tool for functional genomics.BMC plant biology · 2009Article
Corrections and comments
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3 authors.
Funding
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Abstract
The efficiency of Agrobacterium tumefaciens transformation of the model legume Medicago truncatula cv. Jemalong (genotype 2HA) was evaluated for strains LBA 4404, C58pMP90, C58pGV2260 and AGL1. Binary vectors carrying promoter- gus/ gfp reporter gene fusions and the nptII gene as selectable marker were used for plant in vitro transformation/regeneration. The highest transformation efficiency was obtained with the disarmed hypervirulent strain AGL1 (Ti plasmid TiBo542), for which the percentage of explants forming kanamycin (Km)-resistant calli was double that obtained with each of the other three strains. In addition, we were able to reduce the time necessary for plant regeneration using AGL1, with 24% of the explants generating Km-resistant transgenic plantlets within only 4-5 months of culture. Transgene expression in planta was analysed and found to be conserved in the T(1) descendents.
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