ArticleNeurotoxicity research2002
Oxidative stress in rat cortical neurons and astrocytes induced by paraquat in vitro.
Article in Neurotoxicity research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 87 citations in OpenAlex.
- Metabolic partitioning in the brain and its hijacking by glioblastoma.Genes & development · 2023Review
- Proximity to residential and workplace pesticides application and the risk of progression of Parkinson's diseases in Central California.The Science of the total environment · 2023Article
- Role of Oxidative Stress in the Pathogenesis of Amyotrophic Lateral Sclerosis: Antioxidant Metalloenzymes and Therapeutic Strategies.Biomolecules · 2021Review
- Astrocytic Oxidative/Nitrosative Stress Contributes to Parkinson's Disease Pathogenesis: The Dual Role of Reactive Astrocytes.Antioxidants (Basel, Switzerland) · 2019Review
- Astrocytes Do Not Forfeit Their Neuroprotective Roles After Surviving Intense Oxidative Stress.Frontiers in molecular neuroscience · 2019Article
- Heterogeneity of Cells Population and Secretome Profile of Differentiated Cells from E17 Rat Neural Progenitor Cells.Journal of stem cells & regenerative medicine · 2019Article
- Organophosphate pesticide chlorpyrifos impairs STAT1 signaling to induce dopaminergic neurotoxicity: Implications for mitochondria mediated oxidative stress signaling events.Neurobiology of disease · 2018Article
- Local Oxidative Damage in the Soma and Dendrites Quarantines Neuronal Mitochondria at the Site of Insult.iScience · 2018Article
- In vitro model of the blood-brain barrier established by co-culture of primary cerebral microvascular endothelial and astrocyte cells.Neural regeneration research · 2015Article
- Environmental pollutants as risk factors for neurodegenerative disorders: Alzheimer and Parkinson diseases.Frontiers in cellular neuroscience · 2015Review
- Cortical spreading depression increases the phosphorylation of AMP-activated protein kinase in the cerebral cortex.Neurochemical research · 2014Article
- Clinical perspective on oxidative stress in sporadic amyotrophic lateral sclerosis.Free radical biology & medicine · 2013Review
- ROS detoxification and proinflammatory cytokines are linked by p38 MAPK signaling in a model of mature astrocyte activation.PloS one · 2013Article
- Astrocyte mediated protection of fetal cerebral cortical neurons from rotenone and paraquat.Environmental toxicology and pharmacology · 2012Article
- Dopamine and paraquat enhance α-synuclein-induced alterations in membrane conductance.Neurotoxicity research · 2011Article
- Amino acid analog toxicity in primary rat neuronal and astrocyte cultures: implications for protein misfolding and TDP-43 regulation.Journal of neuroscience research · 2011Article
- Glycogen synthase kinase 3β and its phosphorylated form (Y216) in the paraquat-induced model of parkinsonism.Neurotoxicity research · 2011Article
- Molecular mechanisms of pesticide-induced neurotoxicity: Relevance to Parkinson's disease.Chemico-biological interactions · 2010Review
- Increased reactive oxygen species production in the brain after repeated low-dose pesticide paraquat exposure in rats. A comparison with peripheral tissues.Neurochemical research · 2010Article
- The role of integrin alpha(v)beta (8) in neonatal hypoxic-ischemic brain injury.Neurotoxicity research · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oxidative stress has been discussed as crucial mechanism of neuronal cell death in the adult brain. However, it was not clear until now whether neurons are more sensitive to oxidative stress than the other cells in the brain, e.g. astrocytes. Therefore both cell types were exposed to oxidative stress provoked by the redox-cycling compound paraquat. Cortical neurons were found to be more sensitive towards paraquat toxicity than astrocytes as shown by MTT and Neutral Red assay, two different cytotoxicity assays. Mitochondrial functions were determined by the mitochondrial membrane potential and intracellular ATP concentrations. Again cortical neurons were more severely impaired (by paraquat than astrocytes). The production of reactive oxygen species after paraquat exposure was much higher in cortical neurons than in astrocytes and correlated with a higher depletion of GSH (intracellular glutathion). Lipid peroxidation could be shown in astrocytes via the breakdown product malondialdehyde (MDA) whereas in cortical neurons 4-hydroxynonenal (4-HNE) was detected as this endpoint. If and how oxidative stress influences the antioxidant defense was determined via changes in the expression of antioxidant enzymes. Paraquat exposure lead to a 2-3 fold increase of catalase, MnSOD and CuZnSOD mRNA expression in astrocytes. In contrast to astrocytes, in cortical neurons catalase and MnSOD mRNA levels were only marginally elevated above 1.5-fold by treatment with paraquat. Expression levels of glutathione peroxidase (GPx) mRNA were the only one that were not changed in both cell types after paraquat exposure. It is concluded that the more marked increase in expression levels of antioxidant enzymes may render astrocytes more resistant to oxidative stress than neuronal cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.