ArticlePlant molecular biology2003
Rhizosecretion of a monoclonal antibody protein complex from transgenic tobacco roots.
Article in Plant molecular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 87 citations in OpenAlex.
- Improving yield of a recombinant biologic in a Brassica hairy root manufacturing process.Biotechnology and bioengineering · 2022Article
- Assessing Carnivorous Plants for the Production of Recombinant Proteins.Frontiers in plant science · 2019Article
- Rhizosecretion improves the production of Cyanovirin-N in Nicotiana tabacum through simplified downstream processing.Biotechnology journal · 2016Article
- High-yield production of a human monoclonal IgG by rhizosecretion in hydroponic tobacco cultures.Plant biotechnology journal · 2016Article
- Extracellular peptidase hunting for improvement of protein production in plant cells and roots.Frontiers in plant science · 2015Article
- Breakage of transgenic tobacco roots for monoclonal antibody release in an ultra-scale down shearing device.Biotechnology and bioengineering · 2014Article
- Expression of nitrous oxide reductase from Pseudomonas stutzeri in transgenic tobacco roots using the root-specific rolD promoter from Agrobacterium rhizogenes.Ecology and evolution · 2012Article
- Molecular Pharming: future targets and aspirations.Human vaccines · 2011Review
- Different subcellular localization and glycosylation for a functional antibody expressed in Nicotiana tabacum plants and suspension cells.Transgenic research · 2009Article
- Pharma-Planta: road testing the developing regulatory guidelines for plant-made pharmaceuticals.Transgenic research · 2007Review
- Cosecretion of protease inhibitor stabilizes antibodies produced by plant roots.Plant physiology · 2006Article
- Production of an active recombinant thrombomodulin derivative in transgenic tobacco plants and suspension cells.Transgenic research · 2005Article
- Plants as bioreactors: Recent developments and emerging opportunities.Biotechnology advancesReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The secretion of a functional, full-length monoclonal antibody complex from transgenic Nicotiana tabacum roots has been demonstrated. Initially, seeds were germinated on nitrocellulose membranes and antibody secretion detected from the developing roots. Plants were then established in hydroponic culture and secretion into the growth medium measured over 25 days. Western blotting indicated that full-length antibody was present in the medium along with other fragments. Secreted antibody was shown to be functional by binding to antigen in ELISA studies. In contrast, no antibody could be detected from transgenic Nicotiana in which the same antibody was expressed as a membrane protein in the plasmalemma. These results indicate that antibody accumulation in the growth medium is genuinely caused by rhizosecretion and not cell damage. Addition of gelatin to plant growth medium markedly increased levels of antibody accumulation. The mean antibody yield per plant was calculated to be 11.7 microg per gram root dry weight per day. Rhizosecretion may be a viable alternative to agricultural production or cell culture for the generation of monoclonal antibodies in transgenic plants. It may also give rise to novel applications for antibodies expressed in plants such as removal or neutralisation of environmental pollutants and attenuation of pathogens which infect the plant via the rhizosphere.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.