ArticlePlant molecular biology2003
A comprehensive characterization of single-copy T-DNA insertions in the Arabidopsis thaliana genome.
Article in Plant molecular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 202 citations in OpenAlex.
- T-DNA Analyzer: A Long-Read Sequencing Pipeline for Characterizing T-DNA Insertion Sites in Transgenic Crops.International journal of molecular sciences · 2026Article
- Gene Inactivation in Transgenic Plants-A Unique Model for Studying Epigenetic Regulation of Gene Expression.Plants (Basel, Switzerland) · 2026Review
- Genomic consequences associated with Agrobacterium-mediated transformation of plants.The Plant journal : for cell and molecular biology · 2024Review
- Discovery of genes that positively affect biomass and stress associated traits in poplar.Frontiers in plant science · 2024Article
- Glutamine as sole nitrogen source prevents induction of nitrate transporter geneFrontiers in plant science · 2024Article
- The Beneficial FungusInternational journal of molecular sciences · 2023Article
- A perspective from the EU: unintended genetic changes in plants caused by NGT-their relevance for a comprehensive molecular characterisation and risk assessment.Frontiers in bioengineering and biotechnology · 2023Review
- High-throughput detection of T-DNA insertion sites for multiple transgenes in complex genomes.BMC genomics · 2022Article
- Function Analysis of theInternational journal of molecular sciences · 2022Article
- Deficiencies in the Risk Assessment of Genetically Engineered Bt Cowpea Approved for Cultivation in Nigeria: A Critical Review.Plants (Basel, Switzerland) · 2022Review
- Gene targeting in polymerase theta-deficient Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2022Article
- Application of polygene polymerization for insect-resistant poplar breeding.Forestry research · 2022Review
- The Generic Risks and the Potential of SDN-1 Applications in Crop Plants.Plants (Basel, Switzerland) · 2021Review
- Large scale genomic rearrangements in selected Arabidopsis thaliana T-DNA lines are caused by T-DNA insertion mutagenesis.BMC genomics · 2021Article
- Whole-genome resequencing using next-generation and Nanopore sequencing for molecular characterization of T-DNA integration in transgenic poplar 741.BMC genomics · 2021Article
- Datura genome reveals duplications of psychoactive alkaloid biosynthetic genes and high mutation rate following tissue culture.BMC genomics · 2021Article
- Production of Volatile Moth Sex Pheromones in TransgenicBiodesign research · 2021Article
- Foreign Cry1Ab/c Delays Flowering in Insect-Resistant Transgenic Rice via Interaction With Hd3a Florigen.Frontiers in plant science · 2021Article
- Spatiotemporal Controllability and Environmental Risk Assessment of Genetically Engineered Gene Drive Organisms from the Perspective of European Union Genetically Modified Organism Regulation.Integrated environmental assessment and management · 2020Review
- Sequencing of two transgenic early-flowering poplar lines confirmed vector-free single-locus T-DNA integration.Transgenic research · 2020Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
T-DNA flanking sequences were isolated from 112 Arabidopsis thaliana single-copy T-DNA lines and sequence mapped to the chromosomes. Even though two T-DNA insertions mapped to a heterochromatic domain located in the pericentromeric region of chromosome I, expression of reporter genes was detected in these transgenic lines. T-DNA insertion did not seem to be biased toward any of Arabidopsis' five chromosomes. The observed distribution of T-DNA copies in intergenic sequence versus gene sequence (i.e. 5'-upstream regions, coding sequences and 3'-downstream regions) appeared randomly. An evaluation of T-DNA insertion frequencies within gene sequence revealed that integration into 5'-upstream regions occurred more frequently than expected, whereas insertions in coding sequences (exons and introns) were found less frequently than expected based on random distribution predictions. In the majority of cases, single-copy T-DNA insertions were associated with small or large rearrangements such as deletions and/or duplications of target site sequences, deletions and/or duplications of T-DNA sequences, and gross chromosomal rearrangements such as translocations. The accuracy of integration was similarly high for both left- and right-border sequences. These results may be called upon when making detailed molecular analyses of transgenic plants or T-DNA induced mutants.
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Registered trials
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