ArticleAlcoholism, clinical and experimental research2003
Immunohistochemical characterization of hepatic malondialdehyde and 4-hydroxynonenal modified proteins during early stages of ethanol-induced liver injury.
Article in Alcoholism, clinical and experimental research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
- Article
- Unraveling the Gut-Liver-Brain Axis: Microbiome, Inflammation, and Emerging Therapeutic Approaches.Mediators of inflammation · 2025Review
- Lipid peroxidation derived reactive aldehydes in alcoholic liver disease.Current opinion in toxicology · 2019Article
- Aldehyde-Induced DNA and Protein Adducts as Biomarker Tools for Alcohol Use Disorder.Trends in molecular medicine · 2018Review
- Deletion of GSTA4-4 results in increased mitochondrial post-translational modification of proteins by reactive aldehydes following chronic ethanol consumption in mice.Redox biology · 2016Article
- Effects of Resveratrol Supplementation on Oxidative Damage and Lipid Peroxidation Induced by Strenuous Exercise in Rats.Biomolecules & therapeutics · 2015Article
- Increased 4-hydroxynonenal protein adducts in male GSTA4-4/PPAR-α double knockout mice enhance injury during early stages of alcoholic liver disease.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Identification of 5' AMP-activated kinase as a target of reactive aldehydes during chronic ingestion of high concentrations of ethanol.The Journal of biological chemistry · 2014Article
- Proteomic analysis of 4-hydroxynonenal (4-HNE) modified proteins in liver mitochondria from chronic ethanol-fed rats.Redox biology · 2014Article
- Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal.Oxidative medicine and cellular longevity · 2014Review
- Article
- An overview of the chemistry and biology of reactive aldehydes.Free radical biology & medicine · 2013Review
- Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver.Journal of lipid research · 2013Article
- Increased nitroxidative stress promotes mitochondrial dysfunction in alcoholic and nonalcoholic fatty liver disease.Oxidative medicine and cellular longevity · 2013Review
- Protein carbonylation in a murine model for early alcoholic liver disease.Chemical research in toxicology · 2012Article
- A proteomic analysis of liver after ethanol binge in chronically ethanol treated rats.Proteome science · 2012Article
- Overview of lipid peroxidation products and hepatic protein modification in alcoholic liver disease.Chemico-biological interactions · 2011Review
- Chronic ethanol consumption in mice alters hepatocyte lipid droplet properties.Alcoholism, clinical and experimental research · 2011Article
- Markers of oxidative and nitrosative stress in systemic lupus erythematosus: correlation with disease activity.Arthritis and rheumatism · 2010Article
- Using isotopic tools to dissect and quantitate parallel metabolic pathways.Journal of the American Chemical Society · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundChronic ethanol consumption is associated with hepatic lipid peroxidation and the deposition or retention of aldehyde-adducted proteins postulated to be involved in alcohol-induced liver injury. The purpose of this study was to characterize hepatocellular formation of aldehyde-protein adducts during early stages of alcohol-induced liver injury.
methodsFemale Sprague Dawley(R) rats were subjected to the intragastric administration of a low-carbohydrate/high-fat total enteral nutrition diet or a total enteral nutrition diet containing ethanol for a period of 36 days. Indexes of hepatic responses to ethanol were evaluated in terms of changes in plasma alanine aminotransferase activity, hepatic histopathologic analysis, and induction of cytochrome P-4502E1 (CYP2E1). Immunohistochemical methods were used to detect hepatic proteins modified with malondialdehyde (MDA) or 4-hydroxynonenal (4-HNE) for subsequent quantitative image analysis.
resultsAfter 36 days of treatment, rats receiving the alcohol-containing diet displayed hepatic histopathologies characterized by marked micro- and macrosteatosis associated with only minor inflammation and necrosis. Alcohol administration resulted in a 3-fold elevation of plasma alanine aminotransferase activity and 3-fold increases (p < 0.01) in hepatic CYP2E1 apoprotein and activity. Quantitative immunohistochemical analysis revealed significant (p < 0.01) 5-fold increases in MDA- and 4-HNE modified proteins in liver sections prepared from rats treated with alcohol. The MDA- or 4-HNE modified proteins were contained in hepatocytes displaying intact morphology and were colocalized primarily with microvesicular deposits of lipid. Aldehyde-modified proteins were not prevalent in parenchymal or nonparenchymal cells associated with foci of necrosis or inflammation.
conclusionsThese results suggest that alcohol-induced lipid peroxidation is an early event during alcohol-mediated liver injury and may be a sensitizing event resulting in the production of bioactive aldehydes that have the potential to initiate or propagate ensuing proinflammatory or profibrogenic cellular events.
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