ArticleProceedings of the National Academy of Sciences of the United States of America2003
Fluorescence bleaching reveals asymmetric compartment formation prior to cell division in Caulobacter.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 86 citations in OpenAlex.
- Phospho-signaling couples polar asymmetry and proteolysis within a membraneless microdomain in Caulobacter crescentus.Nature communications · 2024Article
- Coupling of cell growth modulation to asymmetric division and cell cycle regulation inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Differential growth regulates asymmetric size partitioning inLife science alliance · 2024Article
- Bacteria-on-a-bead: probing the hydrodynamic interplay of dynamic cell appendages during cell separation.Communications biology · 2022Article
- Modeling the temporal dynamics of master regulators and CtrA proteolysis in Caulobacter crescentus cell cycle.PLoS computational biology · 2022Article
- Computational modeling of unphosphorylated CtrA:iScience · 2021Article
- The DUF1013 protein TrcR tracks with RNA polymerase to control the bacterial cell cycle and protect against antibiotics.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Intracellular amorphous Ca-carbonate and magnetite biomineralization by a magnetotactic bacterium affiliated to the Alphaproteobacteria.The ISME journal · 2021Article
- Absolute Measurements of mRNA Translation in Caulobacter crescentus Reveal Important Fitness Costs of Vitamin BmSystems · 2019Article
- Multilayered control of chromosome replication inBiochemical Society transactions · 2019Review
- An intracellular compass spatially coordinates cell cycle modules in Caulobacter crescentus.Current opinion in microbiology · 2016Review
- Article
- A cell cycle kinase with tandem sensory PAS domains integrates cell fate cues.Nature communications · 2016Article
- Article
- Cell fate regulation governed by a repurposed bacterial histidine kinase.PLoS biology · 2014Article
- Cell cycle-dependent adaptor complex for ClpXP-mediated proteolysis directly integrates phosphorylation and second messenger signals.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- ClpXP and ClpAP proteolytic activity on divisome substrates is differentially regulated following the Caulobacter asymmetric cell division.Molecular microbiology · 2014Article
- Dynamical modeling of the cell cycle and cell fate emergence in Caulobacter crescentus.PloS one · 2014Article
- Coupled protein diffusion and folding in the cell.PloS one · 2014Article
- Computational and genetic reduction of a cell cycle to its simplest, primordial components.PLoS biology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Asymmetric cell division in Caulobacter crescentus yields daughter cells that have different cell fates. Compartmentalization of the predivisional cell is a critical event in the establishment of the differential distribution of regulatory factors that specify cell fate. To determine when during the cell cycle the cytoplasm is compartmentalized so that cytoplasmic proteins can no longer diffuse between the two nascent progeny cell compartments, we designed a fluorescence loss in photobleaching assay. Individual cells containing enhanced GFP were exposed to a bleaching laser pulse tightly focused at one cell pole. In compartmentalized cells, fluorescence disappears only in the compartment receiving the bleaching beam; in noncompartmentalized cells, fluorescence disappears from the entire cell. In a 135-min cell cycle, the cells were compartmentalized 18 +/- 5 min before the progeny cells separated. Clearance of the 22000 CtrA master transcriptional regulator molecules from the stalked portion of the predivisional cell is a controlling element of Caulobacter asymmetry. Monitoring of a fluorescent marker for CtrA showed that the differential degradation of CtrA in the nascent stalk cell compartment occurs only after the cytoplasm is compartmentalized.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.