ArticleNucleic acids research1992
New data concerning the functional organization of the mammalian cell nucleolus: detection of RNA and rRNA by in situ molecular immunocytochemistry.
Article in Nucleic acids research, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 31 citations in OpenAlex.
- The changes in chromosome 6 spatial organization during chromatin polytenization in the Calliphora erythrocephala Mg. (Diptera: Calliphoridae) nurse cells.Protoplasma · 2013Article
- Nucleolar changes and fibrillarin redistribution following apatone treatment of human bladder carcinoma cells.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2010Article
- Methods to detect antifibrillarin antibodies in patients with systemic sclerosis (SSc): a comparison.Journal of clinical laboratory analysis · 2004Article
- Analysis of nucleolar transcription and processing domains and pre-rRNA movements by in situ hybridization.Chromosoma · 1997Article
- Structural changes in oocyte nucleoli of Xenopus laevis during oogenesis and meiotic maturation.Chromosoma · 1996Article
- Non-isotopic electron microscope in situ hybridization for studying the functional sub-compartmentalization of the cell nucleus.Histochemistry and cell biology · 1996Review
- Robert Feulgen Prize Lecture 1995. New approaches to in situ detection of nucleic acids.Histochemistry and cell biology · 1995Review
- Nuclear spreads: I. Visualization of bipartite ribosomal RNA domains.The Journal of cell biology · 1995Article
- The nucleolus.Anatomy and embryology · 1993Review
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated the fine spatial distribution of RNA and rRNA within the Ehrlich tumor cell nucleolus by in situ hybridization with a biotin-labeled probe and by two new strategies, the polyadenylate nucleotidyl transferase-immunogold technique and immuno-labeling with anti-RNA antibodies. Besides the presence, as expected, of RNA and rRNA in the granular component and the dense fibrillar component, we show, for the first time, significant label over all the fibrillar centers of the nucleoli. When RNA and DNA were detected simultaneously on the same sections, only the fibrillar centers were positive for both. These results throw light on the controversial subject of the precise location of transcribing rRNA genes within the nucleolus. The fibrillar centers, and not the dense fibrillar component, should thus be the site of rRNA synthesis.
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