ArticleThe Journal of cell biology2003
The condensin complex is required for proper spindle assembly and chromosome segregation in Xenopus egg extracts.
Article in The Journal of cell biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 53 citations in OpenAlex.
- Making Mitotic Chromosomes in a Test Tube.Epigenomes · 2022Review
- Large domains of heterochromatin direct the formation of short mitotic chromosome loops.eLife · 2020Article
- Comprehensive Discovery of Cell-Cycle-Essential Pathways inThe Plant cell · 2018Article
- Mlh1 deficiency in normal mouse colon mucosa associates with chromosomally unstable colon cancer.Carcinogenesis · 2018Article
- Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis.Journal of cell science · 2018Article
- A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis.Journal of visualized experiments : JoVE · 2015Article
- The condensin complexes play distinct roles to ensure normal chromosome morphogenesis during meiotic division in Arabidopsis.The Plant journal : for cell and molecular biology · 2014Article
- Contrasting roles of condensin I and condensin II in mitotic chromosome formation.Journal of cell science · 2012Article
- Atomic force microscope imaging of chromatin assembled in Xenopus laevis egg extract.Chromosoma · 2011Article
- Xenopus HJURP and condensin II are required for CENP-A assembly.The Journal of cell biology · 2011Article
- Condensins promote chromosome recoiling during early anaphase to complete sister chromatid separation.Developmental cell · 2010Article
- Condensin regulates the stiffness of vertebrate centromeres.Molecular biology of the cell · 2009Article
- The worm solution: a chromosome-full of condensin helps gene expression go down.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Article
- Dissection of the essential steps for condensin accumulation at kinetochores and rDNAs during fission yeast mitosis.The Journal of cell biology · 2008Article
- Condensin function at centromere chromatin facilitates proper kinetochore tension and ensures correct mitotic segregation of sister chromatids.Genes to cells : devoted to molecular & cellular mechanisms · 2007Article
- Disruption of the Arabidopsis SMC4 gene, AtCAP-C, compromises gametogenesis and embryogenesis.Planta · 2006Article
- Characterization and dynamic analysis of Arabidopsis condensin subunits, AtCAP-H and AtCAP-H2.Planta · 2005Article
- Condensin binding at distinct and specific chromosomal sites in the Saccharomyces cerevisiae genome.Molecular and cellular biology · 2005Article
- Histone H1 is essential for mitotic chromosome architecture and segregation in Xenopus laevis egg extracts.The Journal of cell biology · 2005Article
- The Drosophila melanogaster condensin subunit Cap-G interacts with the centromere-specific histone H3 variant CID.Chromosoma · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Chromosome condensation is required for the physical resolution and segregation of sister chromatids during cell division, but the precise role of higher order chromatin structure in mitotic chromosome functions is unclear. Here, we address the role of the major condensation machinery, the condensin complex, in spindle assembly and function in Xenopus laevis egg extracts. Immunodepletion of condensin inhibited microtubule growth and organization around chromosomes, reducing the percentage of sperm nuclei capable of forming spindles, and causing dramatic defects in anaphase chromosome segregation. Although the motor CENP-E was recruited to kinetochores pulled poleward during anaphase, the disorganized chromosome mass was not resolved. Inhibition of condensin function during anaphase also inhibited chromosome segregation, indicating its continuous requirement. Spindle assembly around DNA-coated beads in the absence of kinetochores was also impaired upon condensin inhibition. These results support an important role for condensin in establishing chromosomal architecture necessary for proper spindle assembly and chromosome segregation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.