ArticleThe Journal of physiology1992
A study of the bovine adrenal chromaffin nicotinic receptor using patch clamp and concentration-jump techniques.
Article in The Journal of physiology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 54 citations in OpenAlex.
- New Alpha9 nAChR Ligands Based on a 5-(Quinuclidin-3-ylmethyl)-1,2,4-oxadiazole Scaffold.ACS chemical neuroscience · 2024Article
- Recombinant cellular model system for human muscle-type nicotinic acetylcholine receptor α1Cell stress & chaperones · 2023Article
- α3β4 Acetylcholine Nicotinic Receptors Are Components of the Secretory Machinery Clusters in Chromaffin Cells.International journal of molecular sciences · 2022Article
- Acetylcholine nicotinic receptor subtypes in chromaffin cells.Pflugers Archiv : European journal of physiology · 2018Review
- Unraveling subunit cooperativity in homotetrameric HCN2 channels.Biophysical journal · 2012Article
- Desensitization of neuronal nicotinic receptors of human neuroblastoma SH-SY5Y cells during short or long exposure to nicotine.British journal of pharmacology · 2005Article
- Bimodal action of protons on ATP currents of rat PC12 cells.The Journal of general physiology · 2003Article
- Regulation of the sensitivity of acetylcholine receptors to nicotine in rat habenula neurons.The Journal of physiology · 2000Article
- The gamma-aminobutyric acid type A (GABAA) receptor-associated protein (GABARAP) promotes GABAA receptor clustering and modulates the channel kinetics.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Recovery from open channel block by acetylcholine during neuromuscular transmission in zebrafish.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Regulation of ion channel expression in neural cells by hormones and growth factors.Molecular neurobiology · 1998Review
- A novel slow hyperpolarization-activated potassium current (IK(SHA)) from a mouse hippocampal cell line.The Journal of physiology · 1997Article
- Effects of antimitotic agents on secretion and detergent extractibility of adrenal nicotinic acetylcholine receptors.Cellular and molecular neurobiology · 1997Article
- Influence of subunit composition on desensitization of neuronal acetylcholine receptors at low concentrations of nicotine.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Fading and rebound of currents induced by ATP in PC12 cells.British journal of pharmacology · 1996Article
- Quantal release at a neuronal nicotinic synapse from rat adrenal gland.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Voltage-jump relaxation kinetics for wild-type and chimeric beta subunits of neuronal nicotinic receptors.The Journal of general physiology · 1996Article
- Regions of beta 2 and beta 4 responsible for differences between the steady state dose-response relationships of the alpha 3 beta 2 and alpha 3 beta 4 neuronal nicotinic receptors.The Journal of general physiology · 1995Article
- Acetylcholine-induced currents in acutely dissociated sympathetic neurons from adult hypertensive and normotensive rats have similar properties.Pflugers Archiv : European journal of physiology · 1995Article
- Improved technique for studying ion channels expressed in Xenopus oocytes, including fast superfusion.Biophysical journal · 1994Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. Voltage clamp records have been obtained from bovine adrenal chromaffin cells in the outside-out and whole-cell configurations, in response to step changes of acetylcholine (ACh) concentration. The concentrations used ranged from 50 nM to 20 mM. 2. At high acetylcholine concentrations, the activation and desensitization kinetics of the nicotinic receptor, as observed in outside-out patches, may be described by a model incorporating a single, fast agonist binding step, and relatively slow isomerization to the open state. The affinity of the closed receptor for ACh is 310 microM, the channel opening rate constant is 460 s-1, and the closing rate constant is 29 s-1. 3. Single channel events, observed when nanomolar ACh concentrations are applied to whole cells, have two distinct channel lifetimes: 0.6 ms and 11-15 ms. The variation of the frequencies of the events with ACh concentration, suggests that the short lifetimes are openings of a singly liganded receptor and the longer lifetimes are openings of a doubly liganded receptor. 4. Only a single exponential associated with receptor desensitization is seen with outside-out patches, but two are seen with whole cells. It is postulated that there are two nicotinic receptor types present on adrenal chromaffin cells. 5. The rate of desensitization (9 s-1 and 26 s-1, whole cells; 24 s-1, patches), is fast enough to be significant in determining the open channel lifetime. 6. A sudden increase in current (rebound) is observed when a high concentration of ACh is abruptly removed from outside-out patches. This is evidence for a blocked state. The affinity of the blocking site for ACh is 1400 microM (outside-out patches). 7. The total number of activatable nicotinic channels per whole cell is estimated to be 2600.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.