ArticleThe Biochemical journal2003
Fructose modulates GLUT5 mRNA stability in differentiated Caco-2 cells: role of cAMP-signalling pathway and PABP (polyadenylated-binding protein)-interacting protein (Paip) 2.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- Review
- Late-Stage Functionalization through Click Chemistry Provides GLUT5-Targeting Glycoconjugate as a Potential PET Imaging Probe.International journal of molecular sciences · 2022Article
- Gene variants of the SLC2A5 gene encoding GLUT5, the major fructose transporter, do not contribute to clinical presentation of acquired fructose malabsorption.BMC gastroenterology · 2022Article
- Article
- The Contribution of Dietary Fructose to Non-alcoholic Fatty Liver Disease.Frontiers in pharmacology · 2021Review
- Glucose transporters in the small intestine in health and disease.Pflugers Archiv : European journal of physiology · 2020Review
- Review
- Adenylyl Cyclase 6 Expression Is Essential for Cholera Toxin-Induced Diarrhea.The Journal of infectious diseases · 2019Article
- Circadian rhythm-dependent induction of hepatic lipogenic gene expression in rats fed a high-sucrose diet.The Journal of biological chemistry · 2019Article
- Paip2 cooperates with Cbp80 at an active promoter and participates in RNA Polymerase II phosphorylation in Drosophila.FEBS letters · 2019Article
- Article
- Fructose fuels lung adenocarcinoma through GLUT5.Cell death & disease · 2018Article
- Transporter Gene Expression and Transference of Fructose in Broiler Chick Intestine.The journal of poultry science · 2018Article
- Paip2 is localized to active promoters and loaded onto nascent mRNA in Drosophila.Cell cycle (Georgetown, Tex.) · 2018Article
- The Role of Carbohydrate Response Element Binding Protein in Intestinal and Hepatic Fructose Metabolism.Nutrients · 2017Review
- Fructose Malabsorption in Systemic Sclerosis.Medicine · 2015Observational
- Article
- Regulation of the fructose transporter GLUT5 in health and disease.American journal of physiology. Endocrinology and metabolism · 2008Review
- Regulation of poly(A) binding protein function in translation: Characterization of the Paip2 homolog, Paip2B.RNA (New York, N.Y.) · 2006Article
- Intestinal sugar transport.World journal of gastroenterology · 2006Review
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In intestinal cells, levels of the fructose transporter GLUT5 are increased by glucose and to a greater extent by fructose. We investigated the mechanism by which fructose increases GLUT5 expression. In Caco-2 cells, fructose and glucose increased activity of the -2500/+41 GLUT5 promoter to the same extent. cAMP also activated the GLUT5 promoter. However, if a protein kinase A inhibitor was used to block cAMP signalling, extensive GLUT5 mRNA degradation was observed, with no change in basal transcription levels demonstrating the involvement of cAMP in GLUT5 mRNA stability. Indeed, the half-life of GLUT5 mRNA was correlated ( R2=0.9913) with cellular cAMP levels. Fructose increased cAMP concentration more than glucose, accounting for the stronger effect of fructose when compared with that of glucose on GLUT5 production. We identified several complexes between GLUT5 3'-UTR RNA (where UTR stands for untranslated region) and cytosolic proteins that might participate in mRNA processing. Strong binding of a 140 kDa complex I was observed in sugar-deprived cells, with levels of binding lower in the presence of fructose and glucose by factors of 12 and 6 respectively. This may account for differences in the effects of fructose and glucose. In contrast, the amounts of two complexes of 96 and 48 kDa increased equally after stimulation with either glucose or fructose. Finally, PABP (polyadenylated-binding protein)-interacting protein 2, a destabilizing partner of PABP, was identified as a component of GLUT5 3'-UTR RNA-protein complexes. We conclude that the post-transcriptional regulation of GLUT5 by fructose involves increases in mRNA stability mediated by the cAMP pathway and Paip2 (PABP-interacting protein 2) binding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.