ArticleBiochemistry2003
The three-dimensional structure of bovine calcium ion-bound osteocalcin using 1H NMR spectroscopy.
Article in Biochemistry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 78 citations in OpenAlex.
- Habitual natto intake elevates serum MK-7 levels, enhances osteocalcin carboxylation, and supports bone density: a meta-analysis of Japanese evidence.Frontiers in nutrition · 2025Pooled it
- Update and Reassessment of Data on the Role of Osteocalcin in Bone Properties and Glucose Homeostasis in OC-/- Mice.International journal of molecular sciences · 2025Review
- Lead induced differences in bone properties in osteocalcin +/+ and -/- female mice.Bone reports · 2023Article
- Structural role of osteocalcin and its modification in bone fracture.Applied physics reviews · 2023Article
- Sequential Release of Panax Notoginseng Saponins and Osteopractic Total Flavone from Poly (Journal of functional biomaterials · 2023Article
- Undercarboxylated, But Not Carboxylated, Osteocalcin Suppresses TNF-α-Induced Inflammatory Signaling Pathway in Myoblasts.Journal of the Endocrine Society · 2022Article
- Vitamin K-Dependent Protein Activation: Normal Gamma-Glutamyl Carboxylation and Disruption in Disease.International journal of molecular sciences · 2022Review
- The carboxylation status of osteocalcin has important consequences for its structure and dynamics.Biochimica et biophysica acta. General subjects · 2021Review
- Article
- Growth Factor Engineering Strategies for Regenerative Medicine Applications.Frontiers in bioengineering and biotechnology · 2019Review
- High Fat Mixed Meal Tolerance Test Leads to Suppression of Osteocalcin Decrease in Obese Insulin Resistant Subjects Compared to Healthy Adults.Nutrients · 2018Article
- Structural role of osteocalcin and osteopontin in energy dissipation in bone.Journal of biomechanics · 2018Article
- First evidence of octacalcium phosphate@osteocalcin nanocomplex as skeletal bone component directing collagen triple-helix nanofibril mineralization.Scientific reports · 2018Article
- Vitamin K‑dependent proteins involved in bone and cardiovascular health (Review).Molecular medicine reports · 2018Review
- Biomolecular regulation, composition and nanoarchitecture of bone mineral.Scientific reports · 2018Article
- Orientation and conformation of osteocalcin adsorbed onto calcium phosphate and silica surfaces.Biointerphases · 2017Article
- Carboxylated and undercarboxylated osteocalcin in metabolic complications of human obesity and prediabetes.Diabetes/metabolism research and reviews · 2017Article
- Adsorption of benzoxaboroles on hydroxyapatite phases.Acta biomaterialia · 2016Article
- Evidence for Osteocalcin Binding and Activation of GPRC6A in β-Cells.Endocrinology · 2016Article
- How does the pathophysiological context influence delivery of bone growth factors?Advanced drug delivery reviews · 2015Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Structural information on osteocalcin or other noncollagenous bone proteins is very limited. We have solved the three-dimensional structure of calcium bound osteocalcin using (1)H 2D NMR techniques and proposed a mechanism for mineral binding. The protons in the 49 amino acid sequence were assigned using standard two-dimensional homonuclear NMR experiments. Distance constraints, dihedral angle constraints, hydrogen bonds, and (1)H and (13)C chemical shifts were all used to calculate a family of 13 structures. The tertiary structure of the protein consisted of an unstructured N terminus and a C-terminal loop (residues 16-49) formed by long-range hydrophobic interactions. Elements of secondary structure within residues 16-49 include type III turns (residues 20-25) and two alpha-helical regions (residues 27-35 and 41-44). The three Gla residues project from the same face of the helical turns and are surface exposed. The genetic algorithm-molecular dynamics simulation approach was used to place three calcium atoms on the NMR-derived structure. One calcium atom was coordinated by three side chain oxygen atoms, two from Asp30, and one from Gla24. The second calcium atom was coordinated to four oxygen atoms, two from the side chain in Gla 24, and two from the side chain of Gla 21. The third calcium atom was coordinated to two oxygen atoms of the side chain of Gla17. The best correlation of the distances between the uncoordinated Gla oxygen atoms is with the intercalcium distance of 9.43 A in hydroxyapatite. The structure may provide further insight into the function of osteocalcin.
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Registered trials
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