ArticleInfection and immunity2003
Recombinant soluble CD14 reduces severity of intramammary infection by Escherichia coli.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 72 citations in OpenAlex.
- Selection signatures associated with adaptation in South African Drakensberger, Nguni, and Tuli beef breeds.Tropical animal health and production · 2024Article
- Immune defenses of the mammary gland epithelium of dairy ruminants.Frontiers in immunology · 2022Review
- Ovine CD14- an Immune Response Gene Has a Role Against Gastrointestinal NematodeFrontiers in immunology · 2021Article
- Potential roles of neutrophils in maintaining the health and productivity of dairy cows during various physiological and physiopathological conditions: a review.Immunologic research · 2019Review
- Loss of CD14 leads to disturbed epithelial-B cell crosstalk and impairment of the intestinal barrier after E. coli Nissle monoassociation.Scientific reports · 2018Article
- Sensing of Escherichia coli and LPS by mammary epithelial cells is modulated by O-antigen chain and CD14.PloS one · 2018Article
- Investigating the contribution of IL-17A and IL-17F to the host response during Escherichia coli mastitis.Veterinary research · 2015Article
- Acute Escherichia coli mastitis in dairy cattle: diagnostic parameters associated with poor prognosis.The Journal of veterinary medical science · 2014Article
- The impact of vitamin D in breast cancer: genomics, pathways, metabolism.Frontiers in physiology · 2014Review
- CD14 C-159T polymorphism and its association with chronic lung diseases: A pilot study on isocyanate exposed population of Central India.Indian journal of human genetics · 2013Article
- Immunopathology of mastitis: insights into disease recognition and resolution.Journal of mammary gland biology and neoplasia · 2011Review
- Molecular Characterization and SNP Detection of CD14 Gene of Crossbred Cattle.Molecular biology international · 2011Article
- Endotoxin and CD14 in the progression of biliary atresia.Journal of translational medicine · 2010Article
- Plant production of veterinary vaccines and therapeutics.Current topics in microbiology and immunology · 2009Review
- Bovine CD14 gene characterization and relationship between polymorphisms and surface expression on monocytes and polymorphonuclear neutrophils.BMC genetics · 2008Article
- Human CD14 expressed in seeds of transgenic tobacco displays similar proteolytic resistance and bioactivity with its mammalian-produced counterpart.Transgenic research · 2006Article
- Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.Clinical and diagnostic laboratory immunology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interaction among gram-negative bacteria, the innate immune system, and soluble CD14 (sCD14) has not been well documented. The effect of recombinant bovine sCD14 (rbosCD14) on milk somatic cell count (SCC), bacterial clearance, and cytokine production was investigated by using a bovine intramammary Escherichia coli infection model. We first determined whether rbosCD14 would increase the SCC during a lipopolysaccharide (LPS) challenge. Three quarters of each of six healthy lactating cows were injected with either 0.3 microg of LPS, 0.3 microg of LPS plus 100 micro g of rbosCD14, or saline. In comparison with quarters injected with LPS alone, the SCC was twofold higher (P < 0.05) in quarters injected with LPS plus rbosCD14 after the challenge. We therefore hypothesized that when E. coli bacteria invade the mammary gland, sCD14 in milk would interact with LPS and rapidly recruit neutrophils from the blood to eliminate the bacteria before establishment of infection. To test this hypothesis, two quarters of each of nine healthy cows were challenged with either 50 CFU of E. coli plus saline or 50 CFU of E. coli plus 100 microg of rbosCD14. Quarters challenged with E. coli plus rbosCD14 had a more rapid recruitment of neutrophils, which was accompanied by a faster clearance of bacteria, lower concentrations of tumor necrosis factor alpha and interleukin-8 in milk, and milder clinical symptoms, than challenged quarters injected with saline. Results indicate that increasing the concentration of sCD14 in milk may be a potential strategy with which to prevent or reduce the severity of infection by coliform bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.