ArticleInfection and immunity2003
Host-adapted Borrelia burgdorferi in mice expresses OspA during inflammation.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- Lyme arthritis: linking infection, inflammation and autoimmunity.Nature reviews. Rheumatology · 2021Review
- Laboratory Diagnosis of Lyme Borreliosis.Clinical microbiology reviews · 2021Review
- Case Report: Nuchal Bursitis Associated WithFrontiers in veterinary science · 2021Article
- Detecting Borrelia Spirochetes: A Case Study With Validation Among Autopsy Specimens.Frontiers in neurology · 2021Article
- Late Disseminated Lyme Disease: Associated Pathology and Spirochete Persistence Posttreatment in Rhesus Macaques.The American journal of pathology · 2018Article
- Application of Nanotrap technology for high sensitivity measurement of urinary outer surface protein A carboxyl-terminus domain in early stage Lyme borreliosis.Journal of translational medicine · 2015Article
- Assessment of transcriptional activity of Borrelia burgdorferi and host cytokine genes during early and late infection in a mouse model.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2013Article
- Tick-specific borrelial antigens appear to be upregulated in American but not European patients with Lyme arthritis, a late manifestation of Lyme borreliosis.The Journal of infectious diseases · 2013Article
- Associations of HLA DR and DQ molecules with Lyme borreliosis in Latvian patients.BMC research notes · 2012Article
- Persistence of Borrelia burgdorferi in rhesus macaques following antibiotic treatment of disseminated infection.PloS one · 2012Article
- Anti-Borrelia burgdorferi antibody profile in post-Lyme disease syndrome.Clinical and vaccine immunology : CVI · 2011Article
- OspA-CD40 dyad: ligand-receptor interaction in the translocation of neuroinvasive Borrelia across the blood-brain barrier.Scientific reports · 2011Article
- Toll-like receptors 1 and 2 heterodimers alter Borrelia burgdorferi gene expression in mice and ticks.The Journal of infectious diseases · 2009Article
- Novel Diagnosis of Lyme Disease: Potential for CAM Intervention.Evidence-based complementary and alternative medicine : eCAM · 2009Article
- OspC-independent infection and dissemination by host-adapted Borrelia burgdorferi.Infection and immunity · 2009Article
- Infection of mice with lyme disease spirochetes constitutively producing outer surface proteins a and B.Infection and immunity · 2007Article
- Borrelia burgdorferi intercepts host hormonal signals to regulate expression of outer surface protein A.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Clonal diversification in OspA-specific antibodies from peripheral circulation of a chronic Lyme arthritis patient.Journal of immunological methods · 2007Article
- Identification of specific chemoattractants and genetic complementation of a Borrelia burgdorferi chemotaxis mutant: flow cytometry-based capillary tube chemotaxis assay.Applied and environmental microbiology · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Antibody responses to outer surface protein A (OspA) of Borrelia burgdorferi may occur during periods of arthritis late in the clinical course of untreated Lyme disease. These antibody responses are paradoxical, given the conclusive evidence demonstrating that B. burgdorferi transmitted to the mammalian host expresses little or no OspA. The parallel occurrence of OspA antibodies and arthritic episodes suggests that OspA expression is upregulated during infection with B. burgdorferi. We hypothesized that this was due to the inflammatory environment caused by the immune response to the spirochete. To test our hypothesis, we adapted an in vivo model that mimics the host-pathogen interaction. Dialysis chambers containing B. burgdorferi were implanted into the peritoneal cavities of mice in the presence or absence of zymosan, a yeast cell wall extract that induces inflammation. Spirochetes were harvested 2 days later, and OspA expression was assessed at the protein and transcription level by Western blotting and real-time reverse transcription-PCR, respectively. Flow cytometry was also utilized to evaluate OspA protein expression on individual spirochetes. B. burgdorferi maintained in an inflammatory in vivo environment show an increased OspA expression relative to B. burgdorferi kept under normal in vivo conditions. Furthermore, host-adapted B. burgdorferi with a low OspA phenotype upregulates OspA expression when transferred to an inflammatory in vivo environment. The results obtained by these techniques uniformly identify inflammation as a mediator of in vivo OspA expression in host-adapted B. burgdorferi, providing insights into the behavior of live spirochetes in the mammalian host.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.