ArticleInfection and immunity2003
HEp-2 cell adherence, actin aggregation, and intimin types of attaching and effacing Escherichia coli strains isolated from healthy infants in Germany and Australia.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 59 citations in OpenAlex.
- Molecular characterization ofVeterinary world · 2021Article
- The Prevalence of Shiga Toxin-ProducingInternational journal of microbiology · 2021Article
- Association of Atypical Enteropathogenic Escherichia coli with Diarrhea and Related Mortality in Kittens.Journal of clinical microbiology · 2017Article
- Self-Conjugation of the EnteropathogenicBioMed research international · 2017Article
- Genetic Diversity of Intimin Gene of Atypical Enteropathogenic Escherichia coli Isolated from Human, Animals and Raw Meats in China.PloS one · 2016Article
- Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines.Gut pathogens · 2013Article
- Article
- Specific properties of enteropathogenic Escherichia coli isolates from diarrheal patients and comparison to strains from foods and fecal specimens from cattle, swine, and healthy carriers in Osaka City, Japan.Applied and environmental microbiology · 2013Article
- Clonal relationship among atypical enteropathogenic Escherichia coli strains isolated from different animal species and humans.Applied and environmental microbiology · 2009Article
- Comparative analysis of the locus of enterocyte effacement and its flanking regions.Infection and immunity · 2009Article
- Article
- Characterization of attaching and effacing Escherichia coli (AEEC) isolated from pigs and sheep.BMC microbiology · 2008Article
- Phylogenetic background of attaching and effacing Escherichia coli isolates from animals.Veterinary research communications · 2008Article
- Shiga toxin-mediated hemolytic uremic syndrome: time to change the diagnostic paradigm?PloS one · 2007Article
- Identification of virulence genes linked with diarrhea due to atypical enteropathogenic Escherichia coli by DNA microarray analysis and PCR.Journal of clinical microbiology · 2006Article
- Comparison of virulence gene profiles of Escherichia coli strains isolated from healthy and diarrheic swine.Applied and environmental microbiology · 2006Article
- Bovine feces from animals with gastrointestinal infections are a source of serologically diverse atypical enteropathogenic Escherichia coli and Shiga toxin-producing E. coli strains that commonly possess intimin.Applied and environmental microbiology · 2005Article
- Serotypes, intimin variants and other virulence factors of eae positive Escherichia coli strains isolated from healthy cattle in Switzerland. Identification of a new intimin variant gene (eae-eta2).BMC microbiology · 2005Article
- Escherichia coli and community-acquired gastroenteritis, Melbourne, Australia.Emerging infectious diseases · 2004Article
- Identification of Escherichia coli O114 O-antigen gene cluster and development of an O114 serogroup-specific PCR assay.Journal of clinical microbiology · 2004Article
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fecal samples from healthy children under 2 years of age living in Berlin, Germany (205 infants), and Melbourne, Australia (184 infants), were investigated for the presence of attaching and effacing (AE) Escherichia coli (AEEC) strains by screening for eae (intimin) genes. Twenty-seven AEEC strains were isolated from 14 children (7.6%) from Melbourne and from 12 children (5.9%) from Berlin. The 27 AEEC strains were classified as enterohemorrhagic E. coli (one strain, producing Shiga toxin 1), typical enteropathogenic E. coli (EPEC) (one strain carrying an EPEC adherence factor [EAF] plasmid), and atypical EPEC (25 strains negative for Shiga toxins and EAF plasmids). The AEEC were divided into 18 different serotypes, O-nontypeable and O-rough strains. Typing of their intimin genes revealed the presence of intimin alpha in 6 strains, intimin beta in 11 strains, intimin gamma in 7 strains, intimin zeta in 2 strains, and intimin eta in one strain. Analysis of HEp-2 cell adherence showed diffuse adherence or localized adherence-like patterns in 26 AEEC strains; local adherence was found only with the EAF-positive strain. Ten AEEC strains showed an AE property with the fluorescent actin staining (FAS) test. The introduction of an EAF plasmid (pMAR7) converted 11 FAS-negative AEEC strains to FAS positive and increased the FAS reaction in six FAS-positive AEEC strains, indicating that the genes needed for the AE phenotype were functional in these strains. Our finding indicates that atypical EPEC strains could play a double role as strains that naturally immunize against intimin in humans and as reservoirs for new emerging human pathogenic EPEC strains.
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