ArticleInfection and immunity2003
Antigenic properties of HpaA and Omp18, two outer membrane proteins of Helicobacter pylori.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Review
- Hesperetin Inhibits Expression of Virulence Factors and Growth ofInternational journal of molecular sciences · 2021Article
- Review
- A Novel Multi-Epitopic Peptide Vaccine Candidate AgainstInternational journal of peptide research and therapeutics · 2021Article
- Outer Membrane Vesicles ofFrontiers in microbiology · 2020Article
- Antimicrobial and Anti-Biofilm Effect of an Electrolyzed Superoxidized Solution at Neutral-pH againstBioMed research international · 2019Article
- Synthetic Lipopeptide Enhances Protective Immunity AgainstFrontiers in immunology · 2019Article
- Article
- The rOmp22-HpaA fusion protein confers protective immunity against helicobacter pylori in mice.Current microbiology · 2013Article
- Helicobacter pylori Omp18 and its application in serologic screening of infection.Current microbiology · 2011Article
- Helicobacter pylori and gastric cancer: factors that modulate disease risk.Clinical microbiology reviews · 2010Review
- Helicobacter pylori directs tolerogenic programming of dendritic cells.Gut microbes · 2010Article
- Role of innate immunity in Helicobacter pylori-induced gastric malignancy.Physiological reviews · 2010Review
- Helicobacter pylori: bacterial factors and the role of cytokines in the immune response.Current microbiology · 2010Article
- Diversity of Helicobacter pylori isolates in expression of antigens and induction of antibodies.World journal of gastroenterology · 2008Article
- Helicobacter pylori persistence: an overview of interactions between H. pylori and host immune defenses.Clinical microbiology reviews · 2006Review
- Regulation of murine dendritic cell immune responses by Helicobacter felis antigen.Infection and immunity · 2006Article
- HpaA is essential for Helicobacter pylori colonization in mice.Infection and immunity · 2006Article
- Impact of Helicobacter pylori virulence factors and compounds on activation and maturation of human dendritic cells.Infection and immunity · 2005Article
- Research progress on Helicobacter pylori outer membrane protein.World journal of gastroenterology · 2005Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Outer membrane proteins (OMPs) are incorporated into the outer plasma membrane of Helicobacter pylori and are important for, e.g., ion transport, adherence, structural and osmotic stability, and bacterial virulence but may also be antigenic due to their surface exposure. Previous proteome-based approaches with H. pylori lysates determined a strong serological reaction towards two H. pylori OMPs, HpaA (TIGR HP0797) and Omp18 (TIGR HP1125). PCR was used to detect DNA encoding the two proteins, and a positive signal was found in all H. pylori strains tested. Proteins were cloned and expressed in the human kidney cell line HK293 with the QiaExpressionist system with a C-terminal His tag. Only sera from infected persons showed a positive reaction with the recombinant proteins. Recombinant HpaA (rHpaA) and rOmp18 were incubated with human peripheral blood mononuclear cells and induced secretion of interleukin-12 (IL-12) and IL-10 from these cells. To determine the effect on antigen-presenting cells, human blood monocytic and dendritic cells (DCs) were isolated by magnetic cell separation. rOmp18 and rHpaA strongly stimulated major histocompatibility class II and CD83 expression 7- to 10-fold on isolated DCs. rHpaA and rOmp18 failed to stimulate IL-8 secretion from monocytes but increased secretion of IL-12 and IL-10 from DCs significantly. In summary, HpaA and Omp18 are recognized by human dendritic cells and induce their maturation as well as antigen presentation. HpaA and Omp18 of H. pylori thereby appear to have a specific antigenic potential in humans.
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