ArticleInfection and immunity2003
Cytadherence-deficient mutants of Mycoplasma gallisepticum generated by transposon mutagenesis.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Review
- Designing of novel chimeric PvpA-pMGA protein of Mycoplasma gallisepticum, applicable for indirect ELISA.Journal, genetic engineering & biotechnology · 2022Article
- Genome Editing of Veterinary Relevant Mycoplasmas Using a CRISPR-Cas Base Editor System.Applied and environmental microbiology · 2022Article
- The expression of GapA and CrmA correlates with the Mycoplasma gallisepticum in vitro infection process in chicken TOCs.Veterinary research · 2022Article
- Effects of the In Ovo Vaccination of the ts-11 Strain ofAnimals : an open access journal from MDPI · 2022Article
- A Review ofFrontiers in veterinary science · 2021Review
- Current status of vaccine research, development, and challenges of vaccines for Mycoplasma gallisepticum.Poultry science · 2020Review
- Immunologic Pathways in Protective versus Maladaptive Host Responses to Attenuated and Pathogenic Strains of Mycoplasma gallisepticum.Infection and immunity · 2019Article
- Infection Function of Adhesin-Like Protein ALP609 from Spiroplasma melliferum CH-1.Current microbiology · 2018Article
- Characterization of Mycoplasma gallisepticum pyruvate dehydrogenase alpha and beta subunits and their roles in cytoadherence.PloS one · 2018Article
- Transcriptional Profiling of the Chicken Tracheal Response to Virulent Mycoplasma gallisepticum Strain RInfection and immunity · 2017Article
- Simultaneous Identification of Potential Pathogenicity Factors of Mycoplasma agalactiae in the Natural Ovine Host by Negative Selection.Infection and immunity · 2015Article
- Article
- First identification of proteins involved in motility of Mycoplasma gallisepticum.Veterinary research · 2014Article
- Role of the GapA and CrmA cytadhesins of Mycoplasma gallisepticum in promoting virulence and host colonization.Infection and immunity · 2013Article
- Molecular cloning and characterization of a surface-localized adhesion protein in Mycoplasma bovis Hubei-1 strain.PloS one · 2013Article
- Genome-scale analysis of Mycoplasma agalactiae loci involved in interaction with host cells.PloS one · 2011Article
- Comparative genomic analyses of attenuated strains of Mycoplasma gallisepticum.Infection and immunity · 2010Article
- Unmarked insertional mutagenesis in the bovine pathogen Mycoplasma mycoides subsp. mycoides SC: characterization of a lppQ mutant.Microbiology (Reading, England) · 2008Article
- Transcriptional responses of Mycoplasma gallisepticum strain R in association with eukaryotic cells.Journal of bacteriology · 2007Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytadherence-related molecules of Mycoplasma gallisepticum strain R-low were identified by Tn4001 transposon mutagenesis with the hemadsorption (HA) assay as an indicator for cytadherence. Three Gm(r) HA-negative (HA(-)) colonies displaying a stable HA(-) phenotype through several successive generations in which gentamicin selection was maintained were isolated from four independent transformation experiments and characterized. Southern blot analysis showed that the transposon was inserted as a single copy within the genome of each of the HA(-) mutants, suggesting that the transposon insertion was directly responsible for their inability to attach to erythrocytes. Sequence analysis of the transposon insertion sites revealed that in two mutants, the transposon was inserted at two distinct sites within the gapA structural gene. In the third mutant, the insertion was mapped within the crmA gene, which is located immediately downstream of the gapA gene as part of the same operon. In vitro attachment experiments with the MRC-5 human lung fibroblast cell line showed that the cytadherence capabilities of the HA(-) mutants were less than 25% those of original strain R. Experimental infection of chickens, the natural host of M. gallisepticum, with each of the three mutants demonstrated significantly impaired colonization and host responses. These data demonstrate conclusively the role of both GapA and CrmA proteins in the adherence of M. gallisepticum to host cells in model systems and in vivo colonization. Furthermore, these results underscore the relevance of in vitro cytadherence model systems for studying the pathogenesis of natural infections in chickens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.