ArticleThe American journal of pathology2003
Methylation target array for rapid analysis of CpG island hypermethylation in multiple tissue genomes.
Article in The American journal of pathology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 3 syntheses or guidelines pooled it, 100 citations in OpenAlex.
- Pooled it
- Association of BRCA1 Promoter Methylation with Breast Cancer in Asia: A Meta- AnalysisAsian Pacific journal of cancer prevention : APJCP · 2018Pooled it
- Association of BRCA1 promoter methylation with sporadic breast cancers: Evidence from 40 studies.Scientific reports · 2015Pooled it
- Epigenetic regulation of breast cancer metastasis.Cancer metastasis reviews · 2024Review
- Hybridization-based CpG methylation level detection using methyl-CpG-binding domain-fused luciferase.Analytical and bioanalytical chemistry · 2023Article
- Decreased methylation in the SNAI2 and ADAM23 genes associated with de-differentiation and haematogenous dissemination in breast cancers.BMC cancer · 2018Article
- Methylation-silencing RCC1 expression is associated with tumorigenesis and depth of invasion in gastric cancer.International journal of clinical and experimental pathology · 2015Article
- Kefir improves fatty liver syndrome by inhibiting the lipogenesis pathway in leptin-deficient ob/ob knockout mice.International journal of obesity (2005) · 2014Article
- Methylation-associated gene silencing of RARB in areca carcinogens induced mouse oral squamous cell carcinoma.BioMed research international · 2014Article
- Imprinted genes and satellite loci are differentially methylated in bovine somatic cell nuclear transfer clones.Cellular reprogramming · 2013Article
- Ingestion of milk containing the Dp2 peptide, a dust mite allergen, protects mice from allergic airway inflammation and hyper-responsiveness.Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology · 2013Article
- Inactivation of X-linked tumor suppressor genes in human cancer.Future oncology (London, England) · 2012Review
- Differential differences in methylation status of putative imprinted genes among cloned swine genomes.PloS one · 2012Article
- Comparative methylome analysis of benign and malignant peripheral nerve sheath tumors.Genome research · 2011Article
- DNA methylation: a timeline of methods and applications.Frontiers in genetics · 2011Article
- Gingyo-san enhances immunity and potentiates infectious bursal disease vaccination.Evidence-based complementary and alternative medicine : eCAM · 2011Article
- Granzyme G is expressed in the two-cell stage mouse embryo and is required for the maternal-zygotic transition.BMC developmental biology · 2010Article
- Experimental approaches to the study of epigenomic dysregulation in ageing.Experimental gerontology · 2010Review
- Methods in DNA methylation profiling.Epigenomics · 2009Review
- CpG methylation analysis--current status of clinical assays and potential applications in molecular diagnostics: a report of the Association for Molecular Pathology.The Journal of molecular diagnostics : JMD · 2009Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Hypermethylation of multiple CpG islands is a common event in cancer. To assess the prognostic values of this epigenetic alteration, we developed Methylation Target Array (MTA), derived from the concept of tissue microarray, for simultaneous analysis of DNA hypermethylation in hundreds of tissue genomes. In MTA, linker-ligated CpG island fragments were digested with methylation-sensitive endonucleases and amplified with flanking primers. A panel of 468 MTA amplicons, which represented the whole repertoire of methylated CpG islands in 93 breast tumors, 20 normal breast tissues, and 4 breast cancer cell lines, were arrayed on nylon membrane for probe hybridization. Positive hybridization signals detected in tumor amplicons, but not in normal amplicons, were indicative of aberrant hypermethylation in tumor samples. This is attributed to aberrant sites that were protected from methylation-sensitive restriction and were amplified by PCR in tumor samples, while the same sites were restricted and could not be amplified in normal samples. Hypermethylation frequencies of the 10 genes tested in breast tumors and cancer cell lines were 60% for GPC3, 58% for RASSF1A, 32% for 3OST3B, 30% for HOXA5, 28% for uPA, 25% for WT1, 23% for BRCA1, 9% for DAPK1, and 0% for KL. Furthermore, hypermethylation of 5 to 7 loci of these genes was significantly correlated with hormone receptor status, clinical stages, and ages at diagnosis of the patients analyzed. This novel approach thus provides an additional avenue for assessing clinicopathological consequences of DNA hypermethylation in breast cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.