ArticlePharmaceutical research2003
Apparent lack of Mrp1-mediated efflux at the luminal side of mouse blood-brain barrier endothelial cells.
Article in Pharmaceutical research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Contribution of Direct Cerebral Vascular Transport in Brain Substance Clearance.Aging and disease · 2024Review
- The Interplay of ABC Transporters in Aβ Translocation and Cholesterol Metabolism: Implicating Their Roles in Alzheimer's Disease.Molecular neurobiology · 2021Review
- Non-Human Primate Blood-Brain Barrier and In Vitro Brain Endothelium: From Transcriptome to the Establishment of a New Model.Pharmaceutics · 2020Article
- Culture-induced changes in mRNA expression levels of efflux and SLC-transporters in brain endothelial cells.Fluids and barriers of the CNS · 2020Article
- Mechanisms of glutathione-conjugate efflux from the brain into blood: Involvement of multiple transporters in the course.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2020Article
- ABC Transporters at the Blood-Brain Interfaces, Their Study Models, and Drug Delivery Implications in Gliomas.Pharmaceutics · 2019Review
- Hypoxia-enhanced Blood-Brain Barrier Chip recapitulates human barrier function and shuttling of drugs and antibodies.Nature communications · 2019Article
- Elimination of substances from the brain parenchyma: efflux via perivascular pathways and via the blood-brain barrier.Fluids and barriers of the CNS · 2018Review
- Expression and function of Abcg4 in the mouse blood-brain barrier: role in restricting the brain entry of amyloid-β peptide.Scientific reports · 2017Article
- Uptake and metabolism of sulphated steroids by the blood-brain barrier in the adult male rat.Journal of neurochemistry · 2017Article
- Cellular Models and In Vitro Assays for the Screening of modulators of P-gp, MRP1 and BCRP.Molecules (Basel, Switzerland) · 2017Review
- Role of ABC transporters in the pathogenesis of Alzheimer's disease.ACS chemical neuroscience · 2012Review
- Enhanced brain amyloid-β clearance by rifampicin and caffeine as a possible protective mechanism against Alzheimer's disease.Journal of Alzheimer's disease : JAD · 2012Article
- Direct evidence of abca1-mediated efflux of cholesterol at the mouse blood-brain barrier.Molecular and cellular biochemistry · 2011Article
- PET and SPECT radiotracers to assess function and expression of ABC transporters in vivo.Current drug metabolism · 2011Review
- Physicochemical selectivity of the BBB microenvironment governing passive diffusion--matching with a porcine brain lipid extract artificial membrane permeability model.Pharmaceutical research · 2011Article
- P-glycoprotein deficient mouse in situ blood-brain barrier permeability and its prediction using an in combo PAMPA model.European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences · 2009Article
- Drug interactions at the blood-brain barrier: fact or fantasy?Pharmacology & therapeutics · 2009Review
- Portrait of multifaceted transporter, the multidrug resistance-associated protein 1 (MRP1/ABCC1).Pflugers Archiv : European journal of physiology · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe purpose of this work was to determine mrpl-mediated efflux across the luminal membrane of endothelial cells at the blood-brain barrier (BBB) in mice.
methodsThe transport of radiolabeled etoposide, 17beta-estradiol-D-17beta-glucuronide (E217betaG), vincristine, and doxorubicin across the BBB of mrp1(-/-) and wild-type mice was evaluated by in situ brain perfusion. Etoposide transport was also determined in P-glycoprotein-deficient mdr1a(-/-) mice perfused with both etoposide and mrpl inhibitors like probenecid or MK571. Cerebral vascular volume was determined by co-perfusion with labeled sucrose.
resultsSucrose perfusion indicated that the vascular space was close to normal in all the studies, indicating that the BBB remained intact. The transport of etoposide, E217betaG, vincristine, and doxorubicin into the brain was not affected by the lack of mrp1. Trans-efflux studies in mrp1-deficient mice with etoposide and E217betaG confirmed that mrpl was not involved in the efflux of these substrates across the BBB. There was also a significant P-gp-mediated efflux of etoposide in studies with P-glycoprotein-deficient mdr1a(-/-) mice. Perfusion of mdr1a(-/-) mice etoposide plus probenecid or MK571 did not affect the brain transport of etoposide.
conclusionEfflux mediated by mrp1 does not seem to occur across the luminal membrane of the endothelial cells forming the mouse BBB.
Indexed as
Identifiers
12817896What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.